# US 10,308,984 B2 · Internal control nucleic acids for amplification

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- Number: US 10,308,984 B2
- Document ID: US10308984B2
- Status: Granted
- Kind: B2
- Application number: 15/730,434
- Filed: 2017-10-11
- Published: 2018-04-26
- Granted: 2019-06-04
- Earliest claimed: 2012-03-29
- Assignees: Becton, Dickinson and Company
- Applicants: Becton, Dickinson and Company
- Inventors: Keith Edward Thornton; Danielle Koffenberger; Paul Madepogu
- Web page: https://patentgrep.com/patent/US10308984B2

## CPC

- C12Q 1/6853: using modified primers or templates
- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 2600/166: Oligonucleotides used as internal standards, controls or normalisation probes
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.

## Claims

### Claim 1

A composition comprising:
a polynucleotide comprising a sequence at least 97% identical to the sequence of SEQ ID NO: 1;
a forward primer comprising an oligonucleotide at least 85% identical to SEQ ID NO: 3 or 5;
a reverse primer comprising an oligonucleotide at least 85% identical to SEQ ID NO: 4 or 6;
a single-stranded oligonucleotide probe comprising a detectable moiety attached thereto and a sequence at least 85% identical to SEQ ID NO: 2; and
a sample, wherein the sample does not comprise genomic DNA of the genus Drosophila.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the forward primer is at least 95% identical to SEQ ID NO: 3.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the reverse primer is at least 95% identical to SEQ ID NO: 4.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the forward primer comprises SEQ ID NO: 3, and the reverse primer comprises SEQ ID NO: 4.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the forward primer comprises SEQ ID NO: 5, and the reverse primer comprises SEQ ID NO: 6.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the single-stranded oligonucleotide probe comprises a sequence at least 95% identical to SEQ ID NO: 2.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the single-stranded oligonucleotide probe comprises SEQ ID NO: 2.

### Claim 8 (depends on 1)

The composition of claim 1, wherein the single-stranded oligonucleotide probe further comprises at least one fluorophore, and at least one quencher.

### Claim 9 (depends on 1)

The composition of claim 1, wherein the forward primer is at least 95% identical to SEQ ID NO: 5.

### Claim 10 (depends on 1)

The composition of claim 1, wherein the reverse primer is at least 95% identical to SEQ ID NO: 6.

### Claim 11 (depends on 1)

The composition of claim 1, wherein the sequence of the polynucleotide is not genomic DNA sequence of an organism of the genus Drosophila.

### Claim 12

A kit comprising:
an isolated nucleic acid template for nucleic acid amplification comprising a sequence having at least 97% identity to SEQ ID NO 1;
a first primer comprising an oligonucleotide having at least 85% identity to SEQ ID NO: 3 or 5;
a second primer comprising an oligonucleotide having at least 85% identity to SEQ ID NO: 4 or: 6; and
an oligonucleotide probe comprising a detectable moiety attached thereto and an oligonucleotide comprising a sequence at least 85% identical to SEQ ID NO: 2.

### Claim 13 (depends on 12)

The kit of claim 12, wherein the first primer comprises an oligonucleotide having at least 95% identity to SEQ ID NO: 3 or 5.

### Claim 14 (depends on 12)

The kit of claim 12, wherein the second primer comprises an oligonucleotide having at least 95% identity to SEQ ID NO: 4 or 6.

### Claim 15 (depends on 12)

The kit of claim 12, wherein the first primer comprises SEQ ID NO: 3 or 5.

### Claim 16 (depends on 12)

The kit of claim 12, wherein the second primer comprises SEQ ID NO: 4 or 6.

### Claim 17 (depends on 12)

The kit of claim 12, wherein the first primer comprises SEQ ID NO: 3 and the second primer comprises SEQ ID NO: 4, or wherein the first primer comprises SEQ ID NO: 5 and the second primer SEQ ID NO: 6.

### Claim 18 (depends on 17)

The kit of claim 17, wherein the oligonucleotide probe comprises SEQ ID NO: 2.

### Claim 19 (depends on 12)

The kit of claim 12, wherein the oligonucleotide probe comprises SEQ ID NO: 2.

### Claim 20

An isolated nucleic acid that comprises SEQ ID NO: 1.