# US 10,308,952 B2 · Drought resistance in plants: UPL4

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,308,952 B2
- Document ID: US10308952B2
- Status: Granted
- Kind: B2
- Application number: 14/377,842
- Filed: 2013-02-18
- Published: 2016-01-14
- Granted: 2019-06-04
- Earliest claimed: 2012-02-17
- Assignees: Keygene N.V.
- Applicants: Keygene N.V.
- Inventors: Anne Deslattes Mays; Marieke Helena Adriana Van Hulten; Shital Anilkumar Dixit; Martin De Vos; Jesse David Munkvold; Matthew Vitabile Dileo
- Web page: https://patentgrep.com/patent/US10308952B2

## CPC

- C12N 15/8273: {for drought, cold, salt resistance}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.

## Claims

### Claim 1

A method for identifying one or more plants with drought resistance, comprising determining the sequence of at least part of the endogenous UPL4 gene of said plants; identifying one or more plants having a mutation in the endogenous UPL4 gene resulting in impaired expression of functional UPL4 protein, wherein the functional UPL4 protein comprises the amino acid sequence of any of SEQ ID NO: 2, 6, 8, and 12, wherein the functional UPL4 protein has ubiquitin-protein ligase activity; and exposing the identified one or more plants to drought stress and assaying the plants for drought resistance to select a plant exhibiting improved drought resistance.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the one or more plants are Arabidopsis thaliana.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the one or more plants are Solanum lycopersicum, Brassica rapa, and Oryza sativa.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the one or more plants are part of a heterogenic population.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the heterogenic population is provided by subjecting plant cells to a mutagen that introduces random mutations.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the one or more plants have been subjected to genetic modification targeting the UPL4 gene sequence.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the at least one mutation involves an insertion, a deletion and/or substitution of at least one nucleotide.

### Claim 8 (depends on 1)

The method according to claim 1, wherein impaired expression comprises gene silencing.