# US 10,308,949 B2 · Methods of controlling fructan synthesis in plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,308,949 B2 - Document ID: US10308949B2 - Status: Granted - Kind: B2 - Application number: 15/599,069 - Filed: 2017-05-18 - Published: 2017-11-09 - Granted: 2019-06-04 - Earliest claimed: 2011-08-12 - Assignees: Commonwealth Scientific and Industrial Research Organisation - Applicants: Commonwealth Scientific and Industrial Research Organisation - Inventors: Gangping Xue; Cathrine Lynne McIntyre; Maarten Christiaan Kooiker - Web page: https://patentgrep.com/patent/US10308949B2 ## CPC - C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans} - A21D 13/02: Products made from whole meal; Products containing bran or rough-ground grain - A23L 7/10: Cereal-derived products - C07K 14/415: from plants - C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00) - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} ## Abstract The present invention relates to plants with increased levels of water soluble carbohydrates, particularly fructan, in the stem and leaf sheath. The present invention also provides methods of identifying and/or producing these plants. In particular, the present invention relates to a novel class of polypeptides designated MYB13 which upregulate the expression of enzymes involved in fructan synthesis. The present invention further relates to a novel promoter element that can be used to express genes predominantly in the stem and leaf sheath during the early reproductive stage of a plant. ## Claims ### Claim 1 A transgenic plant comprising an exogenous polynucleotide which encodes a polypeptide with an amino acid sequence that is at least 90% identical to the full length amino acid sequence of any one of SEQ ID NOs 1-4, wherein the polynucleotide is operably linked to a promoter capable of directing expression of the polynucleotide in a cell of the plant. ### Claim 2 (depends on 1) The transgenic plant of claim 1, wherein the exogenous polynucleotide encodes a polypeptide with an amino acid sequence that is at least 95% identical to the full length amino acid sequence of any one of SEQ ID NOs 1-4. ### Claim 3 (depends on 2) The transgenic plant of claim 2, wherein the exogenous polynucleotide encodes a polypeptide with an amino acid sequence that is at least 95% identical to the full length amino acid sequence of SEQ ID NO 1. ### Claim 4 (depends on 2) The transgenic plant of claim 2, wherein the exogenous polynucleotide encodes a polypeptide with an amino acid sequence that is at least 95% identical to the full length amino acid sequence of SEQ ID NO 2. ### Claim 5 (depends on 2) The transgenic plant of claim 2, wherein the exogenous polynucleotide encodes a polypeptide with an amino acid sequence that is at least 95% identical to the full length amino acid sequence of SEQ ID NO 3. ### Claim 6 (depends on 2) The transgenic plant of claim 2, wherein the exogenous polynucleotide encodes a polypeptide with an amino acid sequence that is at least 95% identical to the full length amino acid sequence of SEQ ID NO 4. ### Claim 7 (depends on 1) The transgenic plant of claim 1 which is a cereal plant. ### Claim 8 (depends on 7) The transgenic cereal plant of claim 7, which is wheat, rye, oats, or barley. ### Claim 9 (depends on 1) A seed produced by the transgenic plant of claim 1, wherein said seed comprises the exogenous polynucleotide. ### Claim 10 (depends on 8) A seed produced by the transgenic plant of claim 8, wherein said seed comprises the exogenous polynucleotide. ### Claim 11 (depends on 10) A process for producing flour, wholemeal, starch, a food product or a beverage product, comprising a) obtaining the seed of claim 10; and b) processing the seed to produce the flour, wholemeal, starch, food product or beverage product.