# US 10,308,922 B2 · Methods of processing textiles using polypeptides having endoglucanase activity

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- Number: US 10,308,922 B2
- Document ID: US10308922B2
- Status: Granted
- Kind: B2
- Application number: 15/896,704
- Filed: 2018-02-14
- Published: 2018-06-28
- Granted: 2019-06-04
- Earliest claimed: 2013-10-25
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Weijian Lai; Lan Tang; Marc Dominique Morant; Paul Harris
- Web page: https://patentgrep.com/patent/US10308922B2

## CPC

- C12N 9/2437: {Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)}
- D06M 16/003: {with enzymes or microorganisms}
- C12Y 302/01004: Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
- D06M 2101/04: Vegetal fibres
- D06M 2101/06: cellulosic
- D06M 2200/35: Abrasion, pilling or fibrillation resistance
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- D06M: TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- C12Y: ENZYMES

## Abstract

The present invention relates to polypeptides having endoglucanase activity, catalytic domains, and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

## Claims

### Claim 1

A method for processing a textile, comprising
treating the textile with a polypeptide having endoglucanase activity,
wherein the polypeptide comprises a sequence having at least 95% sequence identity to amino acids 22 to 293 of SEQ ID NO: 2,
and wherein the method is conducted at a temperature in the range of 25-55° C. and at a pH in the range of 5 to 7.5.

### Claim 2 (depends on 1)

The method of claim 1, wherein the polypeptide having endoglucanase activity comprises a sequence having a substitution, deletion, and/or insertion of 1 to 10 amino acids of amino acids 22 to 293 SEQ ID NO: 2.

### Claim 3 (depends on 1)

The method of claim 1, wherein the polypeptide having endoglucanase activity is obtained from a species of the genus Neurospora.

### Claim 4 (depends on 3)

The method of claim 3, wherein the polypeptide having endoglucanase activity is obtained from Neurospora tetrasperma or Neurospora crassa.

### Claim 5 (depends on 1)

The method of claim 1, wherein the polypeptide having endoglucanase activity comprises or consists of SEQ ID NO: 2 or SEQ ID NO: 4.

### Claim 6 (depends on 1)

The method of claim 1, wherein the polypeptide having endoglucanase activity comprises or consists of amino acids 22 to 293 of SEQ ID NO: 2 or amino acids 22 to 293 of SEQ ID NO: 4.

### Claim 7 (depends on 1)

The method of claim 1, wherein the textile is cellulose-containing textile.

### Claim 8 (depends on 1)

The method of claim 1, wherein the textile comprises cotton, viscose, rayon, ramie, linen, or lyocell.

### Claim 9 (depends on 1)

The method of claim 1, wherein the textile comprises synthetic fibers.

### Claim 10 (depends on 1)

The method of claim 1, wherein the method is conducted at a temperature in the range of 30-45° C.

### Claim 11 (depends on 1)

The method of claim 1, wherein the method is conducted at a pH in the range of 6 to 7.

### Claim 12 (depends on 1)

The method of claim 1, wherein the method is conducted at a temperature in the range of 30-45° C. and at a pH in the range of 6 to 7.

### Claim 13 (depends on 1)

The method of claim 1, wherein the polypeptide comprises a sequence having at least 97% sequence identity to amino acids 22 to 293 of SEQ ID NO: 2.

### Claim 14 (depends on 1)

The method of claim 1, wherein the polypeptide comprises a sequence having at least 98% sequence identity to amino acids 22 to 293 of SEQ ID NO: 2.

### Claim 15 (depends on 1)

The method of claim 1, wherein the polypeptide comprises a sequence having at least 99% sequence identity to amino acids 22 to 293 of SEQ ID NO: 2.

### Claim 16 (depends on 1)

The method of claim 1, wherein the polypeptide having endoglucanase activity comprises a sequence having a substitution, deletion, and/or insertion of 1 or 2 amino acids of amino acids 22 to 293 SEQ ID NO: 2.

### Claim 17 (depends on 1)

The method of claim 1, wherein the method is applied in a biopolishing process.

### Claim 18 (depends on 1)

The method of claim 1, wherein the method is applied in a biostoning process.