# US 10,308,907 B2 · Systems for inactivating fluid cultures through heating

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- Number: US 10,308,907 B2
- Document ID: US10308907B2
- Status: Granted
- Kind: B2
- Application number: 15/068,234
- Filed: 2016-03-11
- Published: 2016-07-07
- Granted: 2019-06-04
- Earliest claimed: 2011-01-07
- Assignees: Life Technologies Corporation
- Applicants: Life Technologies Corporation
- Inventors: Jacob D. Lee; Whitt F. Woods
- Web page: https://patentgrep.com/patent/US10308907B2

## CPC

- C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)}
- A61L 2/04: Heat (radiation A61L2/08)
- B01F 27/113: Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
- B01F 27/1134: {the impeller being of hydrofoil type}
- B01F 27/2121: composed of interconnected parts
- B01F 27/213: characterised by the connection with the drive
- B01F 27/88: with a separate receptacle-stirrer unit that is adapted to be coupled to a drive mechanism
- B01F 33/86: {Mixing heads comprising a driven stirrer}
- B01F 35/2215: {Temperature}
- B01F 35/413: {by means of clamps or clamping arrangements for fixing attached stirrers or independent stirrer units}
- B01F 35/451: {by rotating them about an axis parallel to the plane of the opening}
- B01F 35/513: Flexible receptacles, e.g. bags supported by rigid containers
- B01F 35/91: using gas or liquid injected into the material, e.g. using liquefied carbon dioxide or steam
- C12M 23/14: {Bags}
- C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)}
- C12M 23/38: {Caps; Covers; Plugs; Pouring means}
- C12M 23/48: {Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00)}
- C12M 41/24: {inside the vessel}
- C12M 47/20: {Heating or cooling (heating or cooling apparatus for laboratory uses B01L7/00)}
- A61L 2103/05: Living organisms or biological materials
- B01F 2035/99: {Heating}
- B01F 2101/22: Mixing of ingredients for pharmaceutical or medical compositions
- F24H 1/122: {combined with storage tank}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06)
- F24H: FLUID HEATERS, e.g. WATER OR AIR HEATERS, HAVING HEAT-GENERATING MEANS, e.g. HEAT PUMPS, IN GENERAL (steam generation F22)

## Abstract

A fluid heating system includes a tank assembly having an interior surface bounding a chamber, the tank assembly including a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber, and a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening. A collapsible bag is removably disposed within the chamber of the tank assembly, the collapsible bag bounding a compartment adapted to hold a fluid. A mechanism is provided for controlling the temperature of fluid within the collapsible bag when the collapsible bag is positioned within chamber of the tank assembly.

## Claims

### Claim 1

A fluid heating system comprising:
a tank assembly having an interior surface bounding a chamber, the tank assembly comprising:
a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber; and
a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening;
a collapsible bag removably disposed within the chamber of the tank assembly, the collapsible bag bounding a closed compartment adapted to hold a fluid, the compartment being closed when the lid is in the first position and the second position;
means for controlling the temperature of fluid within the collapsible bag when the collapsible bag is positioned within the chamber of the tank assembly; and
a tube coupled with an upper end of the collapsible bag, the tube extending out of the compartment by passing between lid and the sidewall when the lid is in the second position.

### Claim 2 (depends on 1)

The fluid heating system as recited in claim 1, further comprising:
a support projecting from an interior surface of the sidewall so as to be disposed within the chamber; and
a port coupled with the collapsible bag, the port being supported by the support so that the port is disposed a distance from the sidewall.

### Claim 3 (depends on 1)

The fluid heating system as recited in claim 1, further comprising a temperature port assembly coupled to the collapsible bag, the temperature port assembly bounding a cavity that projects into the compartment of the collapsible bag but is not in fluid communication with the compartment.

### Claim 4 (depends on 3)

The fluid heating system as recited in claim 3, further comprising a temperature probe positioned with the cavity of the temperature port assembly.

### Claim 5 (depends on 3)

The fluid heating system as recited in claim 3, further comprising a support extending between the tank assembly and the temperature port assembly, the support supporting the temperature port assembly within the chamber of the tank assembly at a distance from the sidewall.

### Claim 6 (depends on 3)

The fluid heating system as recited in claim 3, wherein the temperature port assembly comprises:
a port secured to the collapsible bag; and
a probe adapter secured to the port, the probe adapter comprising:
a tubular sleeve received over the port;
a mounting flange radially outwardly projecting from the sleeve; and
a receiver coupled to the sleeve and projecting down through the sleeve, the receiver bounding the cavity of the temperature port assembly.

### Claim 7 (depends on 5)

The fluid heating system as recited in claim 5, wherein the support comprises:
a flange mounted to the sidewall of the tank assembly and projecting into the chamber; and
a retainer disposed at an end of the flange, the temperature port assembly being supported on the retainer.

### Claim 8 (depends on 5)

The fluid heating system as recited in claim 5, further comprising a clamp removably securing the support to the temperature port assembly.

### Claim 9 (depends on 1)

The fluid heating system as recited in claim 1, further comprising:
a floor disposed at the second end of the sidewall, a hole extending through the floor; and
a tube coupled with a lower end of the collapsible bag and extending through the hole in the floor.

### Claim 10 (depends on 1)

The fluid heating system as recited in claim 1, wherein the means for controlling the temperature of fluid comprise a fluid channel disposed within the sidewall of the tank assembly.

### Claim 11 (depends on 10)

The fluid heating system as recited in claim 10, further comprising:
a boiler for heating a secondary fluid; and
a pump for pumping the heated secondary fluid from the boiler through the fluid channel within the sidewall of the tank assembly.

### Claim 12 (depends on 1)

The fluid heating system as recited in claim 1, wherein the means for controlling the temperature of fluid comprises:
a fluid channel disposed within the lid of the tank assembly; and
a fluid line fluid coupled to the fluid channel for delivering a secondary fluid into the fluid channel.

### Claim 13

A fluid holding system comprising:
a tank assembly having an interior surface bounding a chamber, the tank assembly comprising a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber; and
a collapsible bag removably disposed within the chamber of the tank assembly and being comprised of one or more sheets of polymeric film, the collapsible bag bounding a compartment adapted to hold a fluid;
a port coupled with the bag and communicating with the compartment; and
a support projecting from an interior surface of the sidewall so as to be completely disposed within the chamber of the tank assembly, the port being supported by the support so that the port is disposed within the chamber at a distance from the sidewall.

### Claim 14 (depends on 13)

The fluid holding system as recited in claim 13, wherein the support comprises a flange projecting from the sidewall and a stem disposed on the flange, the stem at least partially bounding an opening, the port being at least partially disposed within the opening of the stem.

### Claim 15 (depends on 14)

The fluid holding system as recited in claim 14, wherein the stem has a substantially C-shaped transverse cross section.

### Claim 16 (depends on 13)

The fluid holding system as recited in claim 13, further comprising a clamp removably securing the port to the support.

### Claim 17 (depends on 13)

The fluid holding system as recited in claim 13, further comprising a probe adapter secured to the port, the probe adapter comprising:
a tubular sleeve received over the port; and
a mounting flange radially outwardly projecting from the sleeve, the mounting flange being supported on the support.

### Claim 18 (depends on 17)

The fluid holding system as recited in claim 17, further comprising a receiver coupled to the sleeve and projecting down through the sleeve, the receiver bounding a cavity that is at least partially disposed within the compartment of the collapsible bag.

### Claim 19 (depends on 18)

The fluid holding system as recited in claim 18, further comprising a temperature probe positioned with the cavity of the receiver.

### Claim 20 (depends on 13)

The fluid holding system as recited in claim 13, wherein the compartment of the collapsible bag is sterile.

### Claim 21

A fluid heating system comprising:
a tank assembly having an interior surface bounding a chamber, the tank assembly comprising:
a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber; and
a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening;
a collapsible bag removably disposed within the chamber of the tank assembly, the collapsible bag bounding a closed compartment adapted to hold a fluid, the compartment being closed when the lid is in the first position and the second position;
means for controlling the temperature of fluid within the collapsible bag when the collapsible bag is positioned within the chamber of the tank assembly, the means for controlling the temperature of the fluid comprising:
a fluid channel disposed within the lid of the tank assembly; and
a fluid line fluid coupled to the fluid channel for delivering a secondary fluid into the fluid channel
a boiler for heating the secondary fluid; and
a pump for pumping the heated secondary fluid from the boiler, through the fluid line, and to the fluid channel disposed within the lid of the tank assembly.