US 10,308,692 B2 · Variants of GAL2 transporter and their uses

Abstract

The present invention relates to polypeptides which are Gal2 variants comprising at least one amino acid substitution at a position corresponding to M435, and optionally further amino acid substitution(s). The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of bioethanol and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of bioethanol and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing pentose(s), preferably D-xylose and/or L-arabinose.

Claims18

Dependent claims

A polypeptide, comprising at least one amino acid substitution at a position corresponding to M435 and at a position corresponding to N376 of the amino acid sequence of SEQ ID NO:

1,
wherein the polypeptide has at least 80%, at least 90%, or at least 95% sequence identity with the amino acid sequence of SEQ ID NO:
1, and wherein the polypeptide has either or both of an in vitro and an in vivo pentose transport function.

Dependencies are inferred from the claim text.

Facts

Application

15/520,814Filed 22 Oct 2015

Priority

EP 1418992722 Oct 2014

Published

US 2017/0334955 A123 Nov 2017

Granted

4 Jun 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification22 codes

C07K 14/395
From yeasts: from Saccharomyces
C12N 1/18
Yeasts; Culture media therefor: baker's yeast; Brewer's yeast
C12N 9/1205
Transferring phosphorus containing groups, e.g. kinases: phosphotransferases with an alcohol group as acceptor , e.g. protein kinases
C12N 9/90
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: isomerases

Variants of GAL2 transporter and their uses

Life of this patent

Variants of GAL2 transporter and their uses

Life of this patent

Abstract

Claims18