Magnetic separation of lipoproteins using dextran sulfate

A method for purifying lipoproteins suitable for differential charged-particle mobility analysis of lipoprotein class and subclass, said method not including density gradient centrifugation;

• said method comprising:
• combining glycine with a sample comprising lipoproteins to provide a glycine mixture;
• adding one or more cations and dextran sulfate to the glycine mixture to form a precipitation mixture;
• contacting the precipitation mixture with an insoluble solid support, wherein one or more classes or subclasses of lipoproteins from said sample precipitate from said mixture to form precipitated lipoproteins onto said insoluble solid support;
• separating said precipitated lipoproteins on said insoluble solid support from said mixture;
• and
• removing said precipitated lipoproteins from said insoluble solid support;
• wherein said precipitated lipoproteins on said insoluble solid support comprise HDL and one or more selected from the group consisting of LDL, Lp(a), IDL and VLDL;
• wherein said insoluble solid support comprises magnetic beads and binds precipitated lipoproteins without an immunoaffinity agent;
• and
• wherein said dextran sulfate is not biotinylated and said precipitated lipoproteins are not bound to said insoluble solid support by streptavidin.