# US 10,308,561 B2 · Methods and compositions for increasing the amounts of phosphorus available for plant uptake from soils

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,308,561 B2
- Document ID: US10308561B2
- Status: Granted
- Kind: B2
- Application number: 16/004,575
- Filed: 2018-06-11
- Published: 2018-10-11
- Granted: 2019-06-04
- Earliest claimed: 2008-10-01
- Assignees: Novozymes Biologicals, Inc.
- Applicants: Novozymes Biologicals Limited
- Inventors: Garry Lawrence Hnatowich; Shelagh Jean Steckler; Mary Leggett; Kari Priest
- Web page: https://patentgrep.com/patent/US10308561B2

## CPC

- C05F 11/08: Organic fertilisers containing added bacterial cultures, mycelia or the like
- A01C 21/00: Methods of fertilising {, sowing or planting}
- A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom
- A01N 63/36: Penicillium
- C05B 17/00: Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
- C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- A01C: PLANTING; SOWING; FERTILISING (parts, details or accessories of agricultural machines or implements, in general A01B51/00 - A01B75/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C05B: PHOSPHATIC FERTILISERS

## Abstract

The present invention relates to a method of enhancing growth conditions for plants by growing the plants in soil containing, in proximity to the plant roots, both a phosphorus source and at least two strains of the fungus Penicillium, particularly P. bilaiae, more particularly strains NRRL 50162 and NRRL 50169.

## Claims

### Claim 1

A method comprising applying a dry inoculum comprising talc and at least two strains of Penicillium bilaiae to plant seed, wherein application of said plant seed into a soil increases the amount of phosphorous available for plant uptake as compared to introduction of a control seed treated with a control inoculum comprising talc and only one of the at least two strains of Penicillium bilaiae.

### Claim 2 (depends on 1)

The method of claim 1, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50162.

### Claim 3 (depends on 1)

The method of claim 1, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50169.

### Claim 4 (depends on 1)

The method of claim 1, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50170.

### Claim 5 (depends on 1)

The method of claim 1, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50162 and Penicillium bilaiae NRRL 50169.

### Claim 6 (depends on 1)

The method of claim 1, wherein said inoculum is applied to said seed in an amount ranging from 1×102 to 1×106 colony forming units of Penicillium bilaiae per seed.

### Claim 7 (depends on 1)

The method of claim 1, wherein said plant seed is leguminous.

### Claim 8 (depends on 1)

The method of claim 1, wherein said plant seed is selected from the group consisting of alfalfa, bean, chickpea, lentil, pea, peanut, and soybean seed.

### Claim 9 (depends on 1)

The method of claim 1, wherein said plant seed is non-leguminous.

### Claim 10 (depends on 1)

The method of claim 1, wherein said plant seed is selected from the group consisting of barley, corn, oat, rye, sorghum, and wheat seed.

### Claim 11

Plant seed coated with a dry inoculum comprising talc and at least two strains of Penicillium bilaiae wherein application of said plant seed into a soil increases the amount of phosphorous available for plant uptake as compared to introduction of a control seed treated with a control inoculum comprising talc and only one of the at least two strains of Penicillium bilaiae.

### Claim 12 (depends on 11)

The plant seed of claim 11, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50162.

### Claim 13 (depends on 11)

The plant seed of claim 11, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50169.

### Claim 14 (depends on 11)

The plant seed of claim 11, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50170.

### Claim 15 (depends on 11)

The plant seed of claim 11, wherein said inoculum comprises spores of Penicillium bilaiae NRRL 50162 and Penicillium bilaiae NRRL 50169.

### Claim 16 (depends on 11)

The plant seed of claim 11, wherein said plant seed is leguminous.

### Claim 17 (depends on 11)

The plant seed of claim 11, wherein said plant seed is selected from the group consisting of alfalfa, bean, chickpea, lentil, pea, peanut, and soybean seed.

### Claim 18 (depends on 11)

The plant seed of claim 11, wherein said plant seed is non-leguminous.

### Claim 19 (depends on 11)

The plant seed of claim 11, wherein said plant seed is selected from the group consisting of barley, corn, oat, rye, sorghum, and wheat seed.

### Claim 20 (depends on 11)

A method comprising introducing the plant seed of claim 11 into a plant growth medium.