# US 10,307,759 B2 · Biosensor for the detection of target components in a sample > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,307,759 B2 - Document ID: US10307759B2 - Status: Granted - Kind: B2 - Application number: 15/319,068 - Filed: 2015-06-24 - Published: 2017-04-27 - Granted: 2019-06-04 - Earliest claimed: 2014-06-25 - Assignees: Koninklijke Philips N.V. - Applicants: Koninklijke Philips N.V. - Inventors: Albert Hendrik Jan Immink; Jeroen Hans Nieuwenhuis; Gwenola Sabatte; Wilhelmina Maria Hardeman - Web page: https://patentgrep.com/patent/US10307759B2 ## CPC - B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 3/502784: {specially adapted for droplet or plug flow, e.g. digital microfluidics} - G01N 33/54326: {Magnetic particles} - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - B01L 2200/0647: Handling flowable solids, e.g. microscopic beads, cells, particles - B01L 2200/0668: Trapping microscopic beads - B01L 2200/0673: Handling of plugs of fluid surrounded by immiscible fluid - B01L 2200/141: Preventing contamination, tampering - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/087: Multiple sequential chambers - B01L 2400/043: magnetic forces - B01L 2400/0688: surface tension valves, capillary stop, capillary break - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The invention relates to a method and a biosensor system (300) for the detection of target components (TC) in a biological sample. In a preferred embodiment, magnetic purification particles (PP) are added to the sample that can bind interfering components (IC) (if present) which interfere with the detection process. The purification particles (PP) with bound interfering components (IC) may be removed from the sample and transferred through a magneto-capillary valve (MCV1) into a waste chamber (320). ## Claims ### Claim 1 A biosensor system for detection of target components in a sample that comprises interfering components which may interfere with the detection of the target components, comprising: a purification chamber with an inlet for introduction of the sample; purification particles that can bind interfering components of the sample in the purification chamber; a detection-particle storage chamber configured to provide magnetic detection particles, the detection-particle storage chamber comprising a first protrusion that points to a first mirror symmetric protrusion of the purification chamber, wherein the magnetic detection particles bind target components of the sample; wherein the detection particle storage chamber that is connected to the purification chamber via a magneto-capillary valve through which the detection-particles can controllably be moved; and a waste chamber that is connected to the purification chamber via a magneto-capillary valve through which the purification particles can controllably be moved, wherein the waste chamber comprises a second protrusion that points toward a second mirror symmetric protrusion of the purification chamber. ### Claim 2 (depends on 1) The biosensor system according to claim 1, wherein the purification particles are adapted to bind interfering components and/or fractions and/or aggregates chosen from the group consisting essentially of: proteins; antibodies; lipids; nucleic acids; cells; bacteria; enzymes; molecules with a certain resemblance to target analytes that may cross-react; and drugs. ### Claim 3 (depends on 1) The biosensor system according to claim 1, wherein the purification particles comprise surface molecules or atoms and/or fractions and/or aggregates are chosen from the group consisting essentially of: proteins; streptavidin; antibodies; nucleic acids; enzymes; electrically charged molecules or atoms; silica molecules; metal atoms; molecules that may react with interfering components such that their degree of interference is decreased; molecules with a resemblance to target analytes; and capture molecules with an affinity for interfering components. ### Claim 4 (depends on 1) The biosensor system according to claim 1, wherein the magnetic detection particles are provided in the purification chamber and/or in a detection-particle storage chamber prior to the introduction of the sample into the purification chamber. ### Claim 5 (depends on 4) The biosensor system according to claim 4, wherein the detection-particle storage chamber is coupled to the purification chamber via another magneto-capillary valve. ### Claim 6 (depends on 1) The biosensor system according to claim 1, wherein the purification particles are moved by magnetic effect. ### Claim 7 (depends on 1) The biosensor system according to claim 1, wherein the purification particles comprise magnetic particles. ### Claim 8 (depends on 1) The biosensor system according to claim 1, wherein at least one binding spot is provided on a surface of the purification chamber and/or of a separate detection chamber for detection of magnetic detection particles with bound target components. ### Claim 9 (depends on 1) The biosensor system according to claim 1, it further comprises a detection chamber that is coupled to the purification chamber by a magneto-capillary valve allowing for transfer of the magnetic detection particles from the purification chamber to the detection chamber. ### Claim 10 (depends on 1) A method for detection of target components in a sample that comprises interfering components which may interfere with the detection of the target components, the method comprising: providing the biosensor system of claim 1; adding purification particles that can bind interfering components of the sample; transferring said purification particles from a purification chamber through the magneto-capillary valve into a waste chamber; transferring magnetic detection particles that can bind target components to the sample through the magneto-capillary valve into the purification chamber; and detecting said magnetic detection particles with bound target components. ### Claim 11 (depends on 10) The method according to claim 10, wherein the purification particles are added to the sample prior to its introduction into the purification chamber. ### Claim 12 (depends on 10) The method of claim 10, wherein the purification particles are provided in the purification chamber and/or in a purification-particle storage chamber prior to introduction of the sample into the purification chamber. ### Claim 13 (depends on 12) The method according to claim 12, wherein the purification-particle storage chamber is coupled to the purification chamber via a valve, preferably via a magneto-capillary valve. ### Claim 14 (depends on 10) The method according to claim 10, wherein the magnetic detection particles are added to the sample prior to its introduction into the purification chamber.