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US 10,307,474 B2Granted
Modified host cells and hybrid oligosaccharides for use in bioconjugate production
AssigneeGlaxosmithkline Biologicals SA
InventorAmirreza Faridmoayer
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Abstract
Provided herein are host cells capable of producing hybrid oligosaccharides and polysaccharides, wherein said hybrid oligosaccharides and polysaccharides do not comprise a hexose at the reducing end of their first repeat unit. Also provided herein are hybrid oligosaccharides or polysaccharides and bioconjugates which can be produced by the host cells described herein, wherein said bioconjugates comprise a carrier protein linked to a hybrid oligosaccharide or polysaccharide that does not comprise a hexose at the reducing end of its first repeat unit.
Claims10
A hybrid oligosaccharide or polysaccharide having a structure
- (B)n-A→
- a) wherein A is an oligosaccharide repeat unit (i) of a capsular saccharide of a Gram positive bacterial capsular saccharide, a Streptococcus pneumoniae capsular saccharide, (ii) that contains at least 2, 3, 4, 5, 6, 7 or 8 monosaccharides, (iii) with a hexose monosaccharide derivative at the reducing end (indicated by arrow);
- b) wherein B is an oligosaccharide repeat unit (i) containing at least 2, 3, 4, 5, 6, 7 or 8 monosaccharides and (ii) with a hexose monosaccharide at the reducing end of the repeat optionally wherein the hexose monosaccharide comprises glucose, galactose, rhamnose, arabinotol, fucose, or mannose;
- c) wherein A and B are different oligosaccharide repeat units;
- and
- wherein n is at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20.
3 more elements
Dependencies are inferred from the claim text.