# US 10,307,467 B2 · Use of hyaluronidase for treatment of muscle stiffness

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- Number: US 10,307,467 B2
- Document ID: US10307467B2
- Status: Granted
- Kind: B2
- Application number: 15/325,938
- Filed: 2015-07-16
- Published: 2017-07-20
- Granted: 2019-06-04
- Earliest claimed: 2014-07-16
- Assignees: New York University
- Applicants: New York University
- Inventors: Preeti Raghavan; Antonio Stecco
- Web page: https://patentgrep.com/patent/US10307467B2

## CPC

- A61K 38/47: acting on glycosyl compounds (3.2), e.g. cellulases, lactases
- A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)}
- A61K 47/02: Inorganic compounds
- A61P 21/02: Muscle relaxants, e.g. for tetanus or cramps
- C12Y 302/01035: Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12Y: ENZYMES

## Abstract

Provided are methods and kits for reducing the severity of muscle stiffness. The method comprises delivering to one or more specific locations in the deep fascia of an affected muscle a composition comprising a therapeutically effective amount of hyaluronidase.

## Claims

### Claim 1

A method for providing relief from stiffness of a first muscle in an individual comprising the steps of delivering in a region of deep fascia surrounding the first muscle or in a region of deep fascia surrounding a second muscle that affects the function of the first muscle at or near one or more centers of coordination or center of fusion associated with the first muscle, second muscle or a combination thereof, a composition comprising a therapeutically effective amount of hyaluronidase, wherein a center of coordination or center of fusion is characterized by slidable layers in the deep fascia, and wherein delivering the composition results in reduction of stiffness of the first muscle.

### Claim 2 (depends on 1)

The method of claim 1, wherein hyaluronidase is administered via injections at multiple centers of coordination.

### Claim 3 (depends on 2)

The method of claim 2, wherein the amount of hyaluronidase for each injection is about 25 to 300 USP units.

### Claim 4 (depends on 2)

The method of claim 2, wherein the hyaluronidase injections are carried out at 2-6 CC locations.

### Claim 5 (depends on 2)

The method of claim 2, wherein hyaluronidase injections are carried out at 6-10 CC locations.

### Claim 6 (depends on 2)

The method of claim 2, wherein the hyaluronidase injections are repeated over a period of between 1-12 months.