# US 10,307,448 B2 · Method for manufacturing an extract of Rhinacanthus nasutus (L.) kurz

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,307,448 B2
- Document ID: US10307448B2
- Status: Granted
- Kind: B2
- Application number: 15/189,592
- Filed: 2016-06-22
- Published: 2016-10-13
- Granted: 2019-06-04
- Earliest claimed: 2014-06-20
- Assignees: Han Sheng Biotech Co., Ltd.
- Applicants: Han Sheng Biotech Co., Ltd.
- Inventors: Shorong-Shii Liou; I-Min Liu; Chia-Ju Chang; Hou-Ru Ciou; Wei-Fan Chou
- Web page: https://patentgrep.com/patent/US10307448B2

## CPC

- A61K 36/19: Acanthaceae (Acanthus family)
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K 2236/331: using water, e.g. cold water, infusion, tea, steam distillation or decoction
- A61K 2236/333: using mixed solvents, e.g. 70% EtOH
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention discloses a method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz in order to obtain the extract of Rhinacanthus nasutus (L.) Kurz with improved contents of active ingredients, wherein the method comprising the steps of: soaking a raw sample of Rhinacanthus nasutus (L.) Kurz with a processing reagent at 22 to 37° C. for 12 to 36 hours; boiling the soaked product at 1 atm, 95 to 105° C. for 15 minutes to 1 hour to obtain the processed sample of Rhinacanthus nasutus (L.) Kurz; extracting the processed sample of Rhinacanthus nasutus (L.) Kurz by 95% ethanol at 50° C.

## Claims

### Claim 1

A method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz comprising the steps of:
soaking a raw sample of Rhinacanthus nasutus (L.) Kurz with a processing reagent at 22 to 37° C. for 12 to 36 hours;
boiling the soaked product at 1 atm, 95 to 105° C. for 15 minutes to 1 hour to obtain a processed sample of Rhinacanthus nasutus (L.) Kurz; and
extracting the processed sample of Rhinacanthus nasutus (L.) Kurz by 95% ethanol at 50° C.

### Claim 2 (depends on 1)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 1, wherein the method further comprising the step of:
drying the processed sample of Rhinacanthus nasutus (L.) Kurz until the water content of the processed sample of Rhinacanthus nasutus (L.) Kurz is below 10%.

### Claim 3 (depends on 1)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 1, wherein the method further comprising the step of:
before soaking the raw sample of Rhinacanthus nasutus (L.) Kurz with the processing reagent, removing impurities adhering on surfaces of the raw sample of Rhinacanthus nasutus (L.) Kurz.

### Claim 4 (depends on 1)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 1, wherein the processing reagent is wine or vinegar.

### Claim 5 (depends on 4)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 4, wherein 500 grains of the raw sample of Rhinacanthus nasutus (L.) Kurz is soaked with 400 mL of wine.

### Claim 6 (depends on 4)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 4, wherein 500 grams of the raw sample of Rhinacanthus nasutus (L.) Kurz is soaked with 150 mL of vinegar.

### Claim 7 (depends on 4)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 4, wherein the processing reagent is yellow wine.

### Claim 8 (depends on 4)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 4, wherein the processing reagent is rice vinegar.

### Claim 9 (depends on 1)

The method for manufacturing an extract of Rhinacanthus nasutus (L.) Kurz as claimed in claim 1, wherein the raw sample of Rhinacanthus nasutus (L.) Kurz is soaked with the processing reagent for 24 hours.