Method for producing mycosporine-like amino acid using microbes

A method for producing a mycosporine-like amino acid in an extracellular culture solution, the method comprising:

• culturing a microorganism that extracellularly produces a mycosporine-like amino acid, thereby producing the mycosporine-like amino acid in an extracellular culture solution,
• separating the microorganism and the extracellular culture solution, and
• recovering the mycosporine-like amino acid from the extracellular culture solution,
• wherein the microorganism comprises heterologous nucleic acid encoding mycosporine-like amino acid biosynthetic enzymes,
• wherein the heterologous nucleic acid encoding mycosporine-like amino acid biosynthetic enzymes comprises
• a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 1 or a codon-optimized sequence of SEQ ID NO:
• 1, wherein the codon-optimized sequence of SEQ ID NO:
• 1 encodes the same amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 1,
• a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 2 or a codon-optimized sequence of SEQ ID NO:
• 2, wherein the codon-optimized sequence of SEQ ID NO:
• 2 encodes the same amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 2,
• a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 3 or a codon-optimized sequence of SEQ ID NO:
• 3, wherein the codon-optimized sequence of SEQ ID NO:
• 3 encodes the same amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 3, and
• a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 4 or a codon-optimized sequence of SEQ ID NO:
• 4, wherein the codon-optimized sequence of SEQ ID NO:
• 4 encodes the same amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 4,
• wherein codons of the codon-optimized sequences of SEQ ID NOs:
• 1-4 are optimized for expression in the microorganism, and
• wherein the mycosporine-like amino acid is at least one selected from the group consisting of shinorine, porphyra-334, and mycosporine-glycine-alanine.