# US 10,307,347 B2 · Bio-cellulose membrane and method for producing the same

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- Number: US 10,307,347 B2
- Document ID: US10307347B2
- Status: Granted
- Kind: B2
- Application number: 15/412,114
- Filed: 2017-01-23
- Published: 2017-08-10
- Granted: 2019-06-04
- Earliest claimed: 2016-02-05
- Assignees: Tci Co., Ltd.
- Applicants: Tci Co., Ltd
- Inventors: Yung-Hsiang Lin; Cheng-Yu Ho
- Web page: https://patentgrep.com/patent/US10307347B2

## CPC

- A61K 8/0233: {Distinct layers, e.g. core/shell sticks}
- A61K 8/0212: {Face masks}
- A61K 8/731: {Cellulose; Quaternized cellulose derivatives}
- A61Q 19/00: Preparations for care of the skin
- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P 19/12: Disaccharides
- C12P 19/18: produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
- A61K 2800/87: Application Devices; Containers; Packaging
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

A bio cellulose membrane with an outstanding water retention capacity and a method for producing the same are provided. The bio cellulose membrane comprises a first surface, a second surface, and a loose layer, wherein the average pore size of the first surface and the average pore size of the second surface are smaller than that of the loose layer.

## Claims

### Claim 1

A bio cellulose membrane having a water retention capacity, comprising
a first surface, a second surface opposite to the first surface, and a loose layer between the first surface and second surface,
wherein the first surface, the second surface and the loose layer are made of the same material produced from a microbial culture of acetic acid microorganism, the average pore size of the first surface and the average pore size of the second surface independently range from 20 to 80 nm, the average pore size of the loose layer ranges from 50 to 150 nm, and the average pore size of the first surface and the average pore size of the second surface are smaller than that of the loose layer.

### Claim 2 (depends on 1)

A method for producing the bio cellulose membrane of claim 1, comprising:
culturing the acetic acid microorganism with a temperature gradient process such that the microorganism is cultured under the following temperature sequence: a first low temperature, a high temperature, and a second low temperature;
collecting the bio cellulose membrane naturally produced from the culture of the microorganism;
wherein the first low temperature and the second low temperature independently range from 20° C. to 25° C.; and
wherein the high temperature ranges from 30° C. to 35° C.

### Claim 3 (depends on 2)

The method of claim 2, wherein the microorganism is Gluconacetobacter xylinus.

### Claim 4 (depends on 2)

The method of claim 2, wherein the first low temperature and the second low temperature are the same or different.

### Claim 5 (depends on 2)

The method of claim 2, wherein the culture duration at the first low temperature, the culture duration at the high temperature, and the culture duration at the second low temperature are in the ratio of 1:1:2.

### Claim 6 (depends on 5)

The method of claim 5, wherein the culture duration at the first low temperature and the culture duration at the second low temperature are 18 hours respectively.

### Claim 7 (depends on 5)

The method of claim 5, wherein the culture duration at the high temperature is 36 hours.