# US 10,307,207 B2 · Nanoelectroablation control and vaccination > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,307,207 B2 - Document ID: US10307207B2 - Status: Granted - Kind: B2 - Application number: 16/040,434 - Filed: 2018-07-19 - Published: 2018-11-08 - Granted: 2019-06-04 - Earliest claimed: 2014-12-01 - Assignees: Pulse Biosciences, Inc. - Applicants: Pulse Biosciences, Inc. - Inventors: Richard Lee Nuccitelli; Jon Casey Berridge; Zachary Mallon; Mark Kreis; Brian Athos; Pamela Nuccitelli - Web page: https://patentgrep.com/patent/US10307207B2 ## CPC - A61B 1/000094: {extracting biological structures} - A61B 1/00045: {Display arrangement} - A61B 1/043: {for fluorescence imaging} - A61B 1/05: characterised by the image sensor, e.g. camera, being in the distal end portion - A61B 18/1477: {Needle-like probes} - A61B 18/1492: {having a flexible, catheter-like structure, e.g. for heart ablation (A61B18/1477 takes precedence)} - A61B 34/20: Surgical navigation systems; Devices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis - A61B 90/37: {Surgical systems with images on a monitor during operation} - A61N 1/326: {for promoting growth of cells, e.g. bone cells} - A61N 1/40: Applying electric fields by inductive or capacitive coupling (microwave apparatus A61N5/00); {Applying radio-frequency signals} - A61B 1/06: with illuminating arrangements - A61B 6/03: Computed tomography [CT] - A61B 6/12: Arrangements for detecting or locating foreign bodies - A61B 2018/00273: {Anchoring means for temporary attachment of a device to tissue} - A61B 2018/00529: {Liver} - A61B 2018/00577: {Ablation} - A61B 2018/00613: {Irreversible electroporation} - A61B 2018/00642: {with feedback, i.e. closed loop control} - A61B 2018/00892: {Voltage} - A61B 2018/00904: {Automatic detection of target tissue} - A61B 2018/00982: {combined with or comprising means for visual or photographic inspections inside the body, e.g. endoscopes} - A61B 2034/101: {Computer-aided simulation of surgical operations} - A61B 2034/107: {Visualisation of planned trajectories or target regions} - A61M 2037/0007: {having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B) - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} ## Abstract Techniques for treating a tumor and vaccinating against cancer are described. The techniques include treating the tumor by positioning electrodes over an interface between the tumor and non-tumor tissue and applying sub-microsecond pulsed electric fields. The positioning is facilitated by an imaginary contour line of a threshold value of the electric field. In an example, the imaginary contour line is overlaid over images that include the tumor such that the electrodes are properly positioned over the tumor. The techniques also include vaccinating against cancer by passing sub-microsecond pulsed electric fields through tumor cells of a subject sufficient to cause the tumor cells to express calreticulin on surface membranes. The tumor cells are extracted and introduced with the expressed calreticulin into the subject or another subject, thereby providing a vaccination. ## Claims ### Claim 1 A non-transitory storage medium storing instructions that, upon execution on a computing system, cause the computing system to: access an image captured by an imaging device, wherein the image shows at least a portion of an electrode from a set of electrodes, a tumor, and a non-tumor tissue; identify a boundary of the tumor; generate an imaginary contour of a threshold value of an electric field, wherein the electric field is generated based on a voltage between electrodes of the set of electrodes; compare the boundary of the tumor to the imaginary contour; and generate a feedback to align the set of electrodes over the tumor. ### Claim 2 (depends on 1) The medium of claim 1, wherein the feedback is generated on a display device comprising one or more of the following: a fixed display, a heads-up display, a projection display, a near eye display, or glasses. ### Claim 3 (depends on 2) The medium of claim 2, wherein the feedback is presented as a set of instructions on the display device. ### Claim 4 (depends on 1) The medium of claim 1, wherein the feedback comprises a presentation of misalignment between the imaginary contour and the boundary. ### Claim 5 (depends on 1) The medium of claim 1, comprising instructions that cause the computing system to detect whether the set of electrodes is within a threshold distance from the tumor. ### Claim 6 (depends on 1) The medium of claim 1, wherein the imaging device is a CT scanner. ### Claim 7 (depends on 6) The medium of claim 6, comprising instructions that cause the computing system to process CT scans using image processing techniques to detect the boundary of the tumor and/or a location of the set of electrodes. ### Claim 8 (depends on 1) The medium of claim 1, wherein the imaging device is a camera. ### Claim 9 (depends on 1) The medium of claim 1, wherein the imaging device is integrated with a delivery instrument that comprises the set of electrodes. ### Claim 10 (depends on 1) The medium of claim 1, wherein the imaging device is affixed to interactive glasses. ### Claim 11 (depends on 1) The medium of claim 1, wherein the imaginary contour is generated using a 3D model of the electric field. ### Claim 12 (depends on 1) The medium of claim 1, wherein the imaginary contour is generated based on a model of electric field selected from models available from a storage. ### Claim 13 (depends on 12) The medium of claim 12, wherein the model is selected based on one or more of: a configuration of the set of electrodes, a type of the tumor, a size of the tumor, a setting of the electric field, or an application environment. ### Claim 14 (depends on 1) The medium of claim 1, comprising instructions that cause the computing system to recognize a configuration of the set of electrodes. ### Claim 15 (depends on 1) The medium of claim 1, comprising instructions that cause the computing system to detect attachment of the set of electrodes based on a user input or using a pinout of an interface. ### Claim 16 A method of generating a feedback of a location of a delivery instrument, the method comprising: accessing an image captured by an imaging device, wherein the image shows at least a portion of an electrode from a set of electrodes of the delivery instrument, a tumor, and a non-tumor tissue; identifying a boundary of the tumor; generating, using a processor, an imaginary contour of a threshold value of an electric field, wherein the electric field is generated based on a voltage between electrodes of the set of electrodes; comparing the boundary of the tumor to the imaginary contour; and generate a feedback to assist with alignment of the set of electrodes over the tumor. ### Claim 17 (depends on 16) The method of claim 16, the method comprising detecting whether the set of electrodes is within a threshold distance from the tumor. ### Claim 18 (depends on 16) The method of claim 16, the method comprising adjusting the imaginary contour based on one or more of the following: a configuration of the set of electrodes, a type of the tumor, a size of the tumor, a setting of the electric field, or an application environment. ### Claim 19 (depends on 16) The method of claim 16, the method comprising displaying the feedback on a display device. ### Claim 20 A system for generating a feedback of a location of a delivery instrument, the system comprising: a memory storing instructions; and a processor configured to execute the instructions to: access an image captured by an imaging device, wherein the image shows at least a portion of an electrode from a set of electrodes, a tumor, and a non-tumor tissue; identify a boundary of the tumor; generate an imaginary contour of a threshold value of an electric field, wherein the electric field is generated based on a voltage between electrodes of the set of electrodes; compare the boundary of the tumor to the imaginary contour; and generate a feedback to align the set of electrodes over the tumor. ### Claim 21 (depends on 1) The medium of claim 1, wherein the portion of the electrode comprises an end of the electrode. ### Claim 22 (depends on 16) The method of claim 16, wherein the portion of the electrode comprises an end of the electrode. ### Claim 23 (depends on 20) The system of claim 20, wherein the portion of the electrode comprises an end of the electrode.