# US 10,306,895 B2 · Plant defense signaling peptides and applications thereof

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- Number: US 10,306,895 B2
- Document ID: US10306895B2
- Status: Granted
- Kind: B2
- Application number: 15/521,927
- Filed: 2015-10-27
- Published: 2017-11-23
- Granted: 2019-06-04
- Earliest claimed: 2014-10-27
- Assignees: Academia Sinica
- Applicants: Academia Sinica
- Inventors: Yet-Ran Chen; Ying-Lan Chen; Mei-Chun Tseng
- Web page: https://patentgrep.com/patent/US10306895B2

## CPC

- A01N 65/38: Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- C07K 14/415: from plants
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A plant defense signaling peptide and applications thereof for inducing systemic immune responses in a plant. In some embodiments, methods for inducing systemic immune responses in a plant comprises applying to the plant a plant defense signaling polypeptide comprising a motif of SEQ ID NO: 1 or SEQ ID NO: 28, or a composition comprising the polypeptide, wherein the polypeptide has up to 100 amino acids in length.

## Claims

### Claim 1

A method for inducing systemic immune responses in a plant comprising applying to said plant a plant defense signaling polypeptide comprising a motif of SEQ ID NO: 1 or SEQ ID NO: 28, or a composition comprising the polypeptide, wherein the polypeptide has up to 100 amino acids in length.

### Claim 2 (depends on 1)

The method of claim 1, wherein the polypeptide has up to 50 amino acids in length.

### Claim 3 (depends on 1)

The method of claim 1, wherein the polypeptide is selected from the group consisting of SEQ ID NOs: 2-27 and SEQ ID NOs: 29-54.

### Claim 4 (depends on 1)

The method of claim 1, wherein the systemic immune responses include production of hydrogen peroxide (H2O2), generation of a plant hormone and/or expression of an anti-herbivore or anti-pathogen protein.

### Claim 5 (depends on 1)

The method of claim 1, where the polypeptide or the composition is applied to a surface of the plant.

### Claim 6 (depends on 1)

The method of claim 1, where the polypeptide is present in the composition at a concentration of 50 nM or more.

### Claim 7 (depends on 1)

The method of claim 1, wherein the polypeptide or the composition is applied to the plant in an amount effective to induce the systemic immune responses within 2 hours and last for more than 24 hours after the polypeptide or the composition is sprayed to the plant.

### Claim 8 (depends on 1)

The method of claim 1, further comprising treating the plant with salt.

### Claim 9 (depends on 1)

The method of claim 1, wherein the polypeptide has up to 30 amino acids in length.

### Claim 10 (depends on 1)

The method of claim 1, wherein the polypeptide has up to 15 amino acids in length.

### Claim 11 (depends on 1)

The method of claim 1, wherein the polypeptide consists of SEQ ID NO: 1 or SEQ ID NO: 28.

### Claim 12 (depends on 4)

The method of claim 4, wherein the plant hormone is selected from the group consisting of jasmonate (JA), JA conjugated with amino acid isoleucine (JA-Ile), and salicylic acid (SA).

### Claim 13 (depends on 5)

The method of claim 5, wherein the surface of the plant is leaves of the plant.