# US 10,306,893 B2 · Synergistic Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole mixtures for diamondback moth, beet armyworm, sugarcane borer, soybean looper, corn earworm, cabbage looper, and southwestern corn borer control

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- Number: US 10,306,893 B2
- Document ID: US10306893B2
- Status: Granted
- Kind: B2
- Application number: 15/648,095
- Filed: 2017-07-12
- Published: 2017-11-02
- Granted: 2019-06-04
- Earliest claimed: 2015-01-16
- Assignees: Valent BioSciences Corporation
- Applicants: Valent BioSciences LLC
- Inventors: Deanna Branscome; Roger Storey; Russell Eldridge; Emily Brazil; Bala N. Devisetty
- Web page: https://patentgrep.com/patent/US10306893B2

## CPC

- A01N 63/23: B. thuringiensis
- A01N 43/56: 1,2-Diazoles; Hydrogenated 1,2-diazoles
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole for the control of diamondback moth, beet armyworm, sugarcane borer, soybean looper, corn earworm, cabbage looper and southwestern corn borer, wherein the ratio of Bacillus thuringiensis subsp. kurstaki to Bacillus thuringiensis subsp. aizawai is from about 1:0.47 to about 1:0.92, and the ratio of the total amount of Bacillus thuringiensis to chlorantraniliprole is from about 1:0.0001 to about 1:20.

## Claims

### Claim 1

A method of controlling a crop plant pest selected from the group consisting of Plutella spp., Spodoptera spp., Diatraea spp., Chrysodeixis spp., Helicoverpa spp., Trichoplusia spp., and Diatraea spp. comprising applying a pesticidally effective amount of a composition comprising Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole to a plant, wherein the ratio of Bacillus thuringiensis subsp. kurstaki to Bacillus thuringiensis subsp. aizawai is from about 1:0.47 to about 1:0.92, and the ratio of the total amount of Bacillus thuringiensis to chlorantraniliprole is from about 1:0.0001 to about 1:20.

### Claim 2 (depends on 1)

The method of claim 1 wherein the ratio of Bacillus thuringiensis subsp. kurstaki to Bacillus thuringiensis subsp. aizawai is from about 1:0.53 to about 1:0.82.

### Claim 3 (depends on 1)

The method of claim 1 wherein the ratio of the total amount of Bacillus thuringiensis to chlorantraniliprole is from about 1:0.03 to about 1:20.

### Claim 4 (depends on 1)

The method of claim 1 wherein the amount of Bacillus thuringiensis subsp. aizawai is from about 2 to about 160 grams per hectare.

### Claim 5 (depends on 1)

The method of claim 1 wherein the amount of Bacillus thuringiensis subsp. kurstaki is from about 3 to about 240 grams per hectare.

### Claim 6 (depends on 1)

The method of claim 1 wherein the amount of chlorantraniliprole is from about 20 to about 150 grams per hectare.

### Claim 7 (depends on 1)

The method of claim 1 wherein the crop plant pest is a Plutella spp.

### Claim 8 (depends on 1)

The method of claim 1 wherein the crop plant pest is a Spodoptera spp.

### Claim 9 (depends on 1)

The method of claim 1 wherein the crop plant pest is a Diatraea spp.

### Claim 10 (depends on 1)

The method of claim 1 wherein the crop plant pest is a Chrysodeixis spp.

### Claim 11 (depends on 1)

The method of claim 1 wherein the crop plant pest is a Helicoverpa spp.

### Claim 12 (depends on 1)

The method of claim 1 wherein the plant is selected from the group consisting of root and tuber vegetables, bulb vegetables, leafy non-brassica vegetables, leafy brassica vegetables, succulent or dried legumes, fruiting vegetables, cucurbit vegetables, citrus fruits, pome fruits, stone fruits, berry and small fruits, tree nuts, cereal grains, forage and fodder grasses and hay, non-grass animal feeds, herbs, spices, flowers, bedding plants, ornamental flowers, artichoke, asparagus, coffee, cotton, tropical fruits, hops, malanga, peanut, pomegranate, oil seed vegetables, sugarcane, tobacco, turf, and watercress.

### Claim 13 (depends on 12)

The method of claim 12 wherein the plant is genetically modified.

### Claim 14 (depends on 12)

The method of claim 12 wherein the cereal grains are selected from the group consisting of barley, buckwheat, pearl millet, proso millet, oats, corn, field corn, sweet corn, seed corn, popcorn, rice, rye, sorghum (milo), sorghum species, grain sorghum, sudangrass (seed), teosinte, triticale, wheat, wild rice, and cultivars, varieties and hybrids thereof.

### Claim 15 (depends on 14)

The method of claim 14 wherein the plant is genetically modified corn.

### Claim 16 (depends on 12)

The method of claim 12 wherein the succulent or dried vegetable legumes are selected from the group consisting of Lupinus beans, Phaseolus beans, Vigna beans, broad beans, chickpea, guar, jackbean, lablab bean, lentil, Pisum peas, pigeon pea, soybean, immature seed soybean, sword bean, peanut, and cultivars, varieties and hybrids thereof.

### Claim 17 (depends on 16)

The method of claim 16 wherein the plant is genetically modified soybean.
