# US 10,306,892 B2 · Synergistic Bacillus thuringiensis subsp. aizawai and chlorantraniliprole mixtures for diamondback moth, beet armyworm, soybean looper, corn earworm, cabbage looper, and southwestern corn borer control

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- Number: US 10,306,892 B2
- Document ID: US10306892B2
- Status: Granted
- Kind: B2
- Application number: 15/648,004
- Filed: 2017-07-12
- Published: 2017-10-26
- Granted: 2019-06-04
- Earliest claimed: 2015-01-16
- Assignees: Valent BioSciences Corporation
- Applicants: Valent BioSciences LLC
- Inventors: Deanna Branscome; Roger Storey; Russell Eldridge; Emily Brazil
- Web page: https://patentgrep.com/patent/US10306892B2

## CPC

- A01N 63/23: B. thuringiensis
- A01N 43/56: 1,2-Diazoles; Hydrogenated 1,2-diazoles
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai and chlorantraniliprole for the control of Diamondback Moth, Beet Armyworm, Soybean Looper, Corn Earworm, Cabbage Looper, and Southwestern Corn Borer. Specifically, the synergistic ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.00003 to about 1:3.

## Claims

### Claim 1

A method of controlling a crop plant pest selected from the group consisting of Plutella spp., Spodoptera spp., Helicoverpa spp., Trichoplusia spp. and Diatraea spp. comprising applying an effective amount of Bacillus thuringiensis subsp. aizawai and chlorantraniliprole to a plant, wherein the ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.00003 to about 1:3.

### Claim 2 (depends on 1)

The method of claim 1 wherein the ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.001 to about 1:1.

### Claim 3 (depends on 2)

The method of claim 2 wherein the ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.04 to about 1:0.8.

### Claim 4 (depends on 1)

The method of claim 1 wherein the effective amount of Bacillus thuringiensis subsp. aizawai is from about 50 to about 4,000 grams per hectare.

### Claim 5 (depends on 4)

The method of claim 4 wherein the effective amount of Bacillus thuringiensis subsp. aizawai is from about 100 to about 1,300 grams per hectare.

### Claim 6 (depends on 5)

The method of claim 5 wherein the effective amount of Bacillus thuringiensis subsp. aizawai is from about 150 to about 1,250 grams per hectare.

### Claim 7 (depends on 1)

The method of claim 1 wherein the effective amount of chlorantraniliprole is from about 20 to about 150 grams per hectare.

### Claim 8 (depends on 7)

The method of claim 7 wherein the effective amount of chlorantraniliprole is from about 30 to about 130 grams per hectare.

### Claim 9 (depends on 1)

The method of claim 1 wherein the crop plant pest is Plutella spp.

### Claim 10 (depends on 1)

The method of claim 1 wherein the crop plant pest is Spodoptera spp.

### Claim 11 (depends on 1)

The method of claim 1 wherein the crop plant pest is Chrysodeixis spp.

### Claim 12 (depends on 1)

The method of claim 1 wherein the crop plant pest is Helicoverpa spp.

### Claim 13 (depends on 1)

The method of claim 1 wherein the crop plant pest is Trichoplusia spp.

### Claim 14 (depends on 1)

The method of claim 1 wherein the crop plant pest is Diatraea spp.

### Claim 15 (depends on 1)

The method of claim 1 wherein the plant is selected from the group consisting of root and tuber vegetables, bulb vegetables, leafy non-brassica vegetables, leafy brassica vegetables, succulent or dried legumes, fruiting vegetables, cucurbit vegetables, citrus fruits, pome fruits, stone fruits, berry and small fruits, tree nuts, cereal grains, forage and fodder grasses and hay, non-grass animal feeds, herbs, spices, artichoke, asparagus, coffee, cotton, tropical fruits, hops, malanga, peanut, pomegranate, oil seed vegetables, sugarcane, tobacco, and watercress.

### Claim 16 (depends on 15)

The method of claim 15 wherein the plant is genetically modified.

### Claim 17 (depends on 15)

The method of claim 15 wherein the cereal grains are selected from the group consisting of barley, buckwheat, pearl millet, proso millet, oats, corn, field corn, sweet corn, seed corn, popcorn, rice, rye, sorghum (milo), sorghum species, grain sorghum, sudangrass (seed), teosinte, triticale, wheat, wild rice, and cultivars, varieties and hybrids thereof.

### Claim 18 (depends on 17)

The method of claim 17 wherein the plant is genetically modified corn.

### Claim 19 (depends on 15)

The method of claim 15 wherein the succulent or dried vegetable legumes are selected from the group consisting of Lupinus beans, Phaseolus beans, Vigna beans, broad beans, chickpea, guar, jackbean, lablab bean, lentil, Pisum peas, pigeon pea, soybean, immature seed soybean, sword bean, peanut, and cultivars, varieties and hybrids thereof.

### Claim 20 (depends on 19)

The method of claim 19 wherein the plant is genetically modified soybean.
