# US 10,306,891 B2 · Plant growth-promoting microbes, compositions, and uses

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- Number: US 10,306,891 B2
- Document ID: US10306891B2
- Status: Granted
- Kind: B2
- Application number: 15/511,533
- Filed: 2015-09-11
- Published: 2017-08-31
- Granted: 2019-06-04
- Earliest claimed: 2014-09-19
- Assignees: Taxon Biosciences In (Pioneer Pat)
- Applicants: Taxon Biosciences Inc
- Inventors: Matthew N. Ashby; Christopher P. Belnap; Matthew Cornyn Kuchta; Victor Kunin; Caroline Kostecki; Ulrika Lidstrom; Natalia Shestakova; Ladonna Wood
- Web page: https://patentgrep.com/patent/US10306891B2

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- A01N 63/00: Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03)
- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- C12N 1/205: Bacterial isolates
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12R 2001/41: Rhizobium
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present application relates to plant growth promoting microbes (PGPMs), compositions comprising these PGPMs and methods of using these PGPMs and/or compositions for enhancing plant health, plant growth and/or plant yield, and/or for preventing, inhibiting, or treating the development of plant pathogens or the development of phytopathogenic diseases. This application also provides non-naturally occurring plant varieties that are artificially infected with a PGPM descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

## Claims

### Claim 1

A composition comprising a plant seed and one or more microbial strains, wherein at least one microbial strain comprises strain S2373, NRRL Deposit No. B-67109, and wherein the 16S sequence of the strain S2373 comprises any one of SEQ ID Nos: 81, 82, or 83.

### Claim 2 (depends on 1)

The composition of claim 1, further comprising at least one different microbial strain, wherein the 16S sequence of the one different microbial strain comprises any one of SEQ ID Nos: 1-164.

### Claim 3 (depends on 1)

The composition of claim 1, further comprising an agriculturally effective amount of a compound or composition selected from the group consisting of a nutrient, a fertilizer, an acaricide, a bactericide, a fungicide, an insecticide, a microbicide, a nematicide, and a pesticide.

### Claim 4 (depends on 1)

The composition of claim 1, further comprising a carrier.

### Claim 5 (depends on 4)

The composition of claim 4, wherein said carrier is selected from peat, turf, talc, lignite, kaolinite, pyrophyllite, zeolite, montmorillonite, alginate, press mud, sawdust, perlite, mica, silicas, quartz powder, calcium bentonite, vermiculite and mixtures thereof.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the composition is prepared as a formulation selected from the group consisting of an emulsion, a colloid, a dust, a granule, a pellet, a powder, a spray, and a solution.

### Claim 7 (depends on 1)

A plant seed having a coating comprising the composition of claim 1.

### Claim 8 (depends on 1)

The composition of claim 1, wherein the plant seed is a transgenic plant seed.

### Claim 9 (depends on 1)

The composition of claim 1, wherein the composition further comprises a biocontrol agent selected from the group consisting of a bacteria, a fungus, a yeast, a protozoa, a virus, an entomopathogenic nematode, a botanical extract, a protein, a nucleic acid, a secondary metabolite, and an inoculant.