# US 10,306,853 B1 · Resistance alleles in soybean

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- Number: US 10,306,853 B1
- Document ID: US10306853B1
- Status: Granted
- Kind: B1
- Application number: 15/279,031
- Filed: 2016-09-28
- Granted: 2019-06-04
- Earliest claimed: 2015-10-01
- Assignees: Syngenta Participations AG
- Applicants: Syngenta Participations AG
- Inventors: Anderson Rotter Meda; Becky Welsh Breitinger; Flavia Fernandes Carneiro; Andreomar Jose Kurek
- Web page: https://patentgrep.com/patent/US10306853B1

## CPC

- C12Q 1/6895: for plants, fungi or algae
- A01H 1/045: {using molecular markers}
- A01H 5/10: Seeds
- A01H 6/542: {Glycine max [soybean]}
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having resistance Phakosora pachyrhizi. A soybean plant, part thereof and/or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.

## Claims

### Claim 1

A method of producing an Asian Soybean Rust (ASR) tolerant soybean plant or part thereof, the method comprising the steps of:
a. isolating a nucleic acid from a first soybean plant or part thereof;
b. detecting in said nucleic acid, the presence of a marker associated with ASR tolerance in a soybean plant, wherein said marker is located within a chromosomal interval on soybean chromosome 18 corresponding to physical positions 58,722,971-60,802,607 and further wherein the chromosomal interval comprises an allele associated with ASR tolerance, wherein said allele corresponds to an A at position 806 of SEQ ID NO: 2;
c. crossing the first soybean plant having in its genome the marker associated with ASR from b) with a second soybean plant not comprising said marker; and
d. producing a progeny soybean plant from the cross of c) wherein said progeny plant comprises in its genome the marker detected in b).

### Claim 2 (depends on 1)

The method of claim 1, wherein the chromosome interval is derived from any of PI587880A, PI587905, PI561356, PI594538A, or a progeny thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein the marker is a haplotype comprising an A at position 428 of SEQ ID NO: 1; an A at position 806 of SEQ ID NO: 2; an A at position 895 of SEQ ID NO: 3; a G at position 300 of SEQ ID NO: 4; a T at position 301 of SEQ ID NO: 5; an A at position 300 of SEQ ID NO: 6; and an A at position 301 of SEQ ID NO: 7.

### Claim 4 (depends on 1)

The method of claim 1, wherein the chromosomal interval further comprises an allele associated with ASR tolerance that corresponds to: an A at position 428 of SEQ ID NO:1; an A at position 895 of SEQ ID NO:3; a G at position 300 of SEQ ID NO:4; a T at position 301 of SEQ ID NO:5; an A at position 300 of SEQ ID NO:6; an A at position 301 of SEQ ID NO:7; or any combination thereof.