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US 10,301,637 B2Granted
Potatoes with reduced granule-bound starch synthase
AssigneeCellectis
InventorsBenjamin Clasen, Daniel F. Voytas, Feng Zhang
Life of this patent
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Abstract
Materials and methods for making plants (e.g., Solanum varieties) with decreased levels of amylose are provided herein. The methods can include making mutations in the gene encoding granule bound starch synthase (GBSS), where the mutations are induced using a rare-cutting endonuclease.
Claims9
A method for making a Solanum plant, comprising:
- (a) contacting a population of Solanum plant cells comprising a functional GBSS allele with a rare-cutting endonuclease targeted to an endogenous GBSS sequence, wherein said rare-cutting endonuclease is a TALE-nuclease, and wherein said TALE-nuclease binds to a sequence as set forth in SEQ ID NO:2 or SEQ ID NO:3,
- (b) selecting, from said population, a cell in which at least three GBSS alleles have been inactivated, wherein each of said at least three GBSS alleles comprises a deletion of more than one nucleotide base pair, and
- (c) growing said selected plant cell into a Solanum plant, wherein said Solanum plant has reduced levels of amylose as compared to a control Solanum plant in which said at least three GBSS alleles have not been inactivated.
Dependencies are inferred from the claim text.