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US 10,301,349 B2Granted
Dark quenchers for donor-acceptor energy transfer
AssigneeBiosearch Technologies, Inc.
InventorsRonald M. Cook, Matt Lyttle, Daren Dick
Life of this patent
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Abstract
The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
Claims49
A nucleic acid probe comprising a nucleic acid having attached thereto:
- a first quencher moiety of excited state energy having a structure comprising at least three residues selected from substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and combinations thereof, wherein at least two of said residues are covalently linked via an exocyclic diazo bond;
- and
- a second quencher moiety of excited state energy.
Dependencies are inferred from the claim text.