Chloroperoxidase-catalyzed degradation of pharmaceutical pollutants in wastewater
AssigneeThe Florida International University Board of Trustees
InventorsXiaotang Wang, Qinghao He
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Abstract
The present invention provides efficient, economical, and environmentally-friendly compositions and methods for removing pollutants from water sources. In particular embodiments, the present invention provides compositions and methods for catalyzing the degradation of pharmaceutical pollutants in wastewater using the enzyme chloroperoxidase (CPO). Another embodiment provides a method of degrading pollutants in wastewater and other water sources. In specific embodiments, the claimed composition and method can be used to degrade pharmaceutical pollutants selected from the group consisting of: acetaminophen, carbamazepine, sulfamethazine, diclofenac, and naproxen.
Claims18
A reaction mixture composition comprising:
- pharmaceutical pollutants in wastewater, or in another source of water, a remedially-effective amount of chloroperoxidase, an oxidant, a halogen ion, and a phosphate buffer, said remedially effective amount of chloroperoxidase comprising about 1.0 nM to about 50 nM chloroperoxidase, wherein said reaction mixture composition has pH value of about 2.5 to about 3.5.
A reaction mixture composition comprising:
- wastewater, or another water source, comprising:
- chloroperoxidase at a concentration of about 0.1 nM to about 20.0 nM;
- hydrogen peroxide at a concentration of about 0.03 mM to about 2.0 mM;
- a chloride ion at a concentration of about 5.0 mM to about 25 mM;
- and
- a phosphate buffer;
- and
- one or more pollutants selected from acetaminophen, carbamazepine, sulfamethazine, diclofenac, and naproxen present in the wastewater or other water source;
- wherein said reaction mixture composition has a pH value of about 2.5 to about 3.5.
A reaction mixture composition comprising:
- wastewater, or another water source, comprising:
- chloroperoxidase at a concentration of about 20.0 nM to about 50.0 nM;
- hydrogen peroxide at a concentration of about 0.3 mM to about 0.5 mM;
- a chloride ion at a concentration of about 5.0 mM to about 25 mM;
- and
- a phosphate buffer;
- and
- one or more pollutants selected from acetaminophen, carbamazepine, sulfamethazine, diclofenac and naproxen present in the wastewater or other water source;
- wherein said reaction mixture composition has a pH value of about 2.5 to about 3.5.
Dependencies are inferred from the claim text.