Multi-index detection microfluidic chip and methods of use

A microfluidic chip, comprising:

• (1) a bottom plate comprising:
• at least one wave-shaped main channel comprising at least one valley and at least one peak;
• at least one sample inlet port;
• at least one exhaust port;
• and
• at least one reaction chamber,
• wherein one end of said at least one wave-shaped main channel is connected to said at least one sample inlet port, and the other end is connected to said at least one exhaust port,
• wherein said at least one valley points away from the center of said bottom plate, and said at least one peak points toward the center of said bottom plate, and
• wherein said at least one valley of said main channel is connected to said at least one reaction chamber;
• and
• (2) a cover plate fittingly engaging said bottom plate;
• wherein said bottom plate further comprises at least one linking channel, said at least one linking channel connecting said at least one valley of said at least one main channel to said at least one reaction chamber, and wherein said at least one linking channel comprises one or more buffering chambers;
• wherein said at least one main channel forms a plurality of concentric circles on said bottom plate.