Centrifuge tube assembly for separating, concentrating and aspirating constituents of a fluid product
OwnerPatrick Pennieinventor
Life of this patent
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Abstract
A centrifuge tube assembly includes an elongate tubular receptacle having upper and lower ends and a side wall that extends between the ends. A cap attached to the receptacle carries a common inlet/outlet port formed therethrough for communication with an interior chamber of the tubular receptacle. A liquid impermeable piston is longitudinally movable through the receptacle chamber and sealably interengages an interior wall of the receptacle. The piston separates upper and lower regions of the chamber, which respectively communicate with the inlet/outlet port and a pressure neutralizing vent. Biological products are introduced into and aspirated from the receptacle through the inlet/outlet port.
Claims14
A centrifuge tube assembly for separating and aspirating constituent components of a fluid biological product, said assembly comprising:
- an elongate receptacle having an interior chamber for receiving the fluid biological product therein, said receptacle having a lower end and a side wall that extends upwardly from said lower end;
- a cap engaging said side wall and extending across an upper end of said receptacle;
- a common inlet and outlet port formed in one of said cap and said receptacle, said common inlet and outlet port configured for injecting the fluid biological product into said chamber and aspirating the constituent components from said chamber after the constituent components are separated from the fluid biological product;
- a vent formed through said lower end of said receptacle;
- and
- a piston mounted within said chamber and being slidable through said chamber while maintaining sealing engagement with an interior surface of said side wall of said receptacle;
- the fluid biological product being injected through said common port into a region of said chamber between said piston and said upper end of said receptacle and said receptacle being centrifuged to separate the fluid product into constituent components thereof, which components are disposed in respective fluid layers in said chamber, whereby one or more of the fluid layers may be aspirated through said common inlet and outlet port, said vent neutralizing pressure within said receptacle chamber as fluid is injected into said chamber and as constituent components are aspirated from said chamber through said common inlet and outlet port;
- said piston consisting of a cylindrical element having a generally circular peripheral shape conforming to the interior shape of the side wall of said receptacle, said piston being driven toward said lower end of said receptacle exclusively in response to hydraulic pressure exerted by fluid biological product injected into said chamber and toward said upper end of said receptacle exclusively in response to suction created in said chamber by aspiration of the constituent components from said chamber.
A method of separating fluid biological product into constituent components using a centrifuge assembly, which includes a receptacle having upper and lower ends and a side wall that extends between said upper and lower ends, a common inlet and outlet port formed in said upper end of the receptacle and configured for injecting fluid biological product into said receptacle and aspirating constituent components from said receptacle, a vent formed through said lower end and a piston mounted for sliding within the receptacle while maintaining sealing engagement with the side wall of thereof, said method comprising:
- injecting the fluid biological product into the receptacle through the common port into a region of the chamber between the piston and the upper end of the receptacle such that the hydraulic pressure of the injected fluid drives said piston downwardly through the receptacle and until said receptacle is filled to a selected level by the biological product;
- closing said common inlet and outlet port;
- centrifuging said receptacle to separate the fluid biological product into constituent components;
- opening said common inlet and outlet port;
- and
- aspirating one or more of the constituent components from said receptacle chamber through said common inlet and outlet port such that a suction is generated in said receptacle chamber and said sealing piston is drawn upwardly through said receptacle, said vent neutralizing pressure within said receptacle chamber as fluid is introduced into said chamber and as constituent components are aspirated from said chamber.
Dependencies are inferred from the claim text.