Method for generating an affinity reagent library
AssigneesQueen Mary University of London, University of Kent
InventorsAhuva Nissim, John Marshall, Mark Howard
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Abstract
The present disclosure provides a method for generating an affinity reagent library against a target protein which interacts with a ligand, which comprises the following steps; •i) determining one or more structural element(s) of the ligand which are involved in ligand: target protein interaction; •ii) producing a library of peptides which retain these structural element(s); and •iii) grafting each peptide from the library of peptides into a portion of the affinity reagent molecule such that it may interact with the target protein, in order to produce an affinity reagent library.
Claims17
A method for generating an affinity reagent library against a target protein which interacts with a ligand, wherein the target protein is αVβ6, and wherein the method comprises:
- i) generating a library of peptides which comprise:
- a hairpin which contains, at its turn, an RGD motif;
- an α-helix or a 310-helix located C-terminal to the hairpin;
- and
- a LQEKGI (SEQ. ID NO:
- 34)C-terminal capping motif;
- and
- ii) grafting each peptide from the library of peptides into a portion of the affinity reagent molecule such that it may interact with the target protein, in order to produce an affinity reagent library.
A peptide comprising the amino acid sequence shown as SEQ ID NO:
- 23 or SEQ ID NO:
- 31.
Dependencies are inferred from the claim text.