Method and device for depositing a substance on a submerged surface

A method for depositing a substance on a submerged deposition surface, comprising:

• obtaining a spotter, including:
• an outlet cavity defined at least in part by a spotting orifice, a first opening, and a second opening,
• a first conduit fluidly coupled to the first opening, and
• a second conduit fluidly coupled to the second opening;
• sealing the spotting orifice against a deposition surface;
• causing a fluid comprising a deposition material to flow through the first conduit into the outlet cavity and out through the second conduit, thereby communicating among the first opening, the second opening, and the deposition surface to form a deposition spot comprising the deposition material on the deposition surface via the spotting orifice sealed against the deposition surface;
• and
• removing the spotting orifice from the deposition surface, wherein the deposition surface is submerged and the spotter is at least partially submerged in a liquid prior to removing the spotting orifice from the deposition surface such that the liquid covers the deposition spot when the spotting orifice is removed from the deposition surface, wherein the liquid is not the fluid comprising the deposition material.