Extemporaneous preparation of autologous fibrin

A removable single use device for treatment and/or isolation of plasma or blood components, the device comprising an apparatus configured for enriching a fibrinogen content of a plasma fraction, the apparatus comprising:

• interconnected chambers comprising a filtering chamber, a first expandable chamber and a second expandable chamber, each of the interconnected chambers fitted with an inlet and/or an outlet, the apparatus further comprising at least one transfer means selected from the group consisting of (i) means for controlling a flow of fluids and (ii) means for moving the fluids, the at least one transfer means configured to transfer the fluids from one of the interconnected chambers to another one of the interconnected chambers or to the outside of the device;
• and
• a circuit loop comprising the interconnected chambers, a first tubing leading to the inlet of the filtering chamber, a second tubing extending from the outlet of the filtering chamber, and a plurality of valves comprising a first valve, a second valve, a third valve and a fourth valve, the second tubing comprising the third and fourth valves,
• wherein the first valve is configured to selectively connect to a first inlet of the device, the second expandable chamber, the first tubing and the second tubing,
• wherein the filtering chamber is provided with internal filtering means configured to separate water initially present in the plasma fraction from the plasma fraction, and the plurality of valves are configured for controlling the flow of the plasma fraction along the circuit loop such that the circuit loop can allow multiple passes of the plasma fraction over the filtering means,
• wherein the first expandable chamber is connected to the filtering chamber by the second valve, and the second valve directs the water initially present in the plasma fraction from the filtering chamber to the first expandable chamber, and
• the second expandable chamber is connected to the circuit loop and the filtering chamber to establish a closed circuit between the filtering chamber the first expandable chamber and the second expandable chamber.