Autophagy-inducing peptide analogs
AssigneeBoard of Regents, The University of Texas System
InventorsBeth C. Levine, Sanae Shoji-Kawata, Nick V. Grishin, Lisa N. Kinch, Olivier Lichtarge, Angela D. Wilkins
Life of this patent
Estimating the term…
Abstract
An autophagy-inducing compound comprises an autophagy-inducing peptide comprising Beclin 1 peptides immediately N- and C-terminally flanked by moieties R1 and R2, respectively, wherein up to six of said peptide residues may be substituted, R1 and R2 do not naturally flank the Beclin 1 residues, and F270 and F274 are optionally substituted and optionally linked. The compounds may be used to induce autophagy.
Claims20
An autophagy-inducing compound comprising an autophagy-inducing 9, 10 or 11-mer peptide of Beclin 1, and no more of Beclin 1 than the 9, 10 or 11-mer peptide, the peptide comprising Beclin 1 residue 269 or 270 to Beclin 1 residue 278 or 279, wherein Beclin 1 residues 269-279 is VFNATFHIWHS (SEQ ID NO:01), wherein up to six of said residues may be substituted, and wherein:
- (a) F270 and F274 are substituted or linked;
- (b) the peptide is cyclized;
- (c) the peptide is N-terminally flanked by moiety R1, and R1 comprises a transduction domain, a homing peptide, or a serum stabilizing agent;
- or
- (d) the peptide comprises a sequence, unsubstituted, selected from:
- Peptide
- Sequence
- SEQ ID NO
- Mutant 1
- CFNATFEIWHD
- SEQ ID NO:
- 10;
- Mutant 2
- VWNATFEIWHD
- SEQ ID NO:
- 11;
- Mutant 3
- VFDATFEIWHD
- SEQ ID NO:
- 12;
- Mutant 4
- VFNSTFEIWHD
- SEQ ID NO:
- 13;
- Mutant 5
- VFNACFEIWHD
- SEQ ID NO:
- 14;
- Mutant 6
- VFNATWEIWHD
- SEQ ID NO:
- 15;
- Mutant 7
- VFNATFDIWHD
- SEQ ID NO:
- 16;
- Mutant 8
- VFNATFELWHD
- SEQ ID NO:
- 17;
- Mutant 9
- VFNATFEIFHD
- SEQ ID NO:
- 18;
- Mutant 10
- VFNATFEIWYD
- SEQ ID NO:
- 19;
- Mutant 11
- VFNATFEIWHE
- SEQ ID NO:
- 20;
- Mutant 12
- VWNATFELWHD
- SEQ ID NO:
- 21;
- Mutant 13
- VYNATFEIWHD
- SEQ ID NO:
- 22;
- Mutant 14
- VFNATFEVWHD
- SEQ ID NO:
- 23;
- Mutant 15
- VLNATFEIWHD
- SEQ ID NO:
- 24;
- Mutant 16
- VFNATFEMWHD
- SEQ ID NO:
- 25;
- Mutant 17
- VWNATFHIWHD
- SEQ ID NO:
- 26;
- Mutant 18
- VFNATFEFWHD
- SEQ ID NO:
- 27;
- Mutant 19
- VFNATFEYWHD
- SEQ ID NO:
- 28;
- Mutant 20
- VFNATFERWHD
- SEQ ID NO:
- 29;
- Mutant 21
- FNATFEIWHD
- SEQ ID NO:
- 30;
- Mutant 25
- VFNATFEIWH
- SEQ ID NO:
- 31;
- Mutant 26
- FNATFEIWH
- SEQ ID NO:
- 32;
- Mutant 27
- WNATFHIWH
- SEQ ID NO:
- 33;
- Mutant 28
- VWNATFHIWH
- SEQ ID NO:
- 34;
- and
- Mutant 29
- WNATFHIWHD
- SEQ ID NO:
- 35.
Dependencies are inferred from the claim text.