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US 10,300,091 B2Granted
Sustained release angiogenesis modulating compositions and methods for induction and modulation of angiogenesis
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsPeter S. McFetridge, Marc C. Moore
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Abstract
The present disclosure provides compositions including a human placental extract and biodegradable microparticles, sustained release angiogenesis-modulating compositions, compositions and methods for releasing a placental extract to a target over a period of time, and methods for inducing and/or modulating angiogenesis and identifying modulators of angiogenesis. The present disclosure also provides methods of making a composition, including a placental extract that can induce and/or modulate angiogenesis.
Claims29
A composition comprising:
- a human placental extract obtained from a human placental sample, wherein blood and solids have been substantially removed from the extract, and the extract comprises placental proteins including cytokines and growth factors, wherein the placental proteins were present in the placental sample, and the extract comprises less than 2% normalized protein abundance of collagen IV, less than 2% normalized protein abundance of laminin, or less than 2% normalized protein abundance of each of collagen IV and laminin;
- and
- biodegradable microparticles,
- wherein the human placental extract is coupled to the microparticles such that the human placental extract is released from the microparticles over a period of time after exposure to cells in vivo or in vitro.
1 more element
A method of inducing angiogenesis comprising:
- contacting cells with a sustained release angiogenesis-modulating composition, the sustained release angiogenesis-modulating composition comprising:
- biodegradable microparticles, and
- a human placental extract coupled to the microparticles such that the human placental extract is released from the microparticles over a period of time after exposure of the microparticles to cells in vivo or in vitro, wherein the placental extract comprises an extract obtained from a human placental sample, wherein blood and solids have been substantially removed from the extract, and the extract comprises placental proteins including cytokines and growth factors, wherein the placental proteins were present in the placental sample, and wherein the extract comprises less than 2% normalized protein abundance of collagen IV, less than 2% normalized protein abundance of laminin, or less than 2% normalized protein abundance of each of collagen IV and laminin.
A method of reducing inflammation in a subject comprising:
- administering to the subject a composition comprising biodegradable microparticles and a human placental extract obtained from a human placental sample, wherein blood and solids have been substantially removed from the extract, and the extract comprises placental proteins including cytokines and growth factors, wherein the placental proteins were present in the placental sample, and wherein the extract comprises less than 2% normalized protein abundance of collagen IV, less than 2% normalized protein abundance of laminin, or less than 2% normalized protein abundance of each of collagen IV and laminin,
- wherein the human placental extract is coupled to the microparticles such that the human placental extract is released from the microparticles over a period of time after exposure to cells.
A composition comprising:
- biodegradable microparticles;
- and
- a human placental extract coupled to the microparticles such that the human placental extract is released from the microparticle over a period of time after exposure of the microparticles to cells in vivo or in vitro, wherein the placental extract comprises an extract obtained from a human placental sample and made by:
- removing blood from a sample obtained from the human placental sample to produce a crude extract;
- mixing the crude placental extract with a protein solubilization agent to solubilize proteins in the crude extract;
- separating solid materials from the solubilized protein-placental extract mixture;
- performing dialysis on the solubilized protein-placental extract mixture to remove the protein solubilization agent from the mixture;
- and
- after dialysis, removing polymerized solids from the extract to produce the human placental extract,
- wherein the placental extract includes at least 20 different cytokines;
- and
- wherein the placental extract increases angiogenesis.
9 more elements
Dependencies are inferred from the claim text.