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US 10,300,089 B2Granted
Methods for high scale therapeutic production of memory NK cells
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsAlicja J. Copik, Robert Y. Igarashi, Jeremiah L. Oyer, Deborah Altomare
Life of this patent
Estimating the term…
Abstract
Disclosed are compositions and methods relating to the expansion of memory NK cells.
Claims19
A method for increasing the number of memory Natural Killer (NK) cells comprising a) preactivating NK cells by contacting at least one NK cell with at least one or more stimulatory cytokines;
- and b) expanding the preactivated NK cells of step a) by contacting said cells with plasma membrane (PM) particles with surface bound interleukin (IL)-21 (PM21) particles, exosomes (EX) with surface bound IL-21 (EX21) exosomes, or feeder cells (FC) with surface bound IL-21 (FC21) feeder cells;
- wherein the NK cells are further contacted with stimulatory peptides 4-1BB ligand (4-1BBL) and IL-21.
Dependencies are inferred from the claim text.