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US 10,299,940 B2Granted
Method and device for producing a three-dimensional, multi-cell object
AssigneeTechnische UniversitäT Berlin
InventorLutz Kloke
Life of this patent
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Abstract
A method for producing a three-dimensional, multi-cell object, in which polymerized structures are produced in layers by the irradiation of light radiation. To this end, use is made of photopolymerizable liquids which at least partly comprise biological cells. The invention also relates to a device for carrying out such a method and to the use of this device for producing an artificial organ.
Claims11
A method for producing a three-dimensional, multi-cell object, comprising the following steps:
- a) introducing a first photopolymerizable liquid into a reaction vessel,
- b) focusing a first light beam on first selected regions of a first focal plane that lies within an area of the reaction vessel filled with the first liquid,
- c) producing a first polymerized structure in a first layer in the reaction vessel by way of the first light beam in the selected regions,
- d) discharging uncured first photopolymerizable liquid from the reaction vessel,
- e) introducing a further photopolymerizable liquid into the reaction vessel, so the area outside of the previously produced polymerized structure first focal plane is filled with the further photopolymerizable liquid,
- f) focusing a further light beam on the focal plane that lies within an area of the reaction vessel filled with the further liquid,
- g) producing a further polymerized structure in a further layer in the reaction vessel by way of the further light beam, wherein the further polymerized structure is arranged directly adjacent the previously produced polymerized structure and is connected thereto, and
- h) raising the polymerized structure the thickness of one layer and repeating steps d) through g) with the same or different photopolymerizable liquids until the three-dimensional multi-cell object is produced,
- wherein the first photopolymerizable liquid and/or at least one of the further photopolymerizable liquids contain biological cells;
- characterized in that more than one polymerized structure is produced in the same layer.
7 more elements
Dependencies are inferred from the claim text.