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US 10,299,707 B2Granted
Compact biosensor of matrix metalloproteinase with cadmium free quantum dots
AssigneeStc.Unm
InventorsJohn B. Plumley, Erin D. Milligan, Marek Osinski
Life of this patent
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Abstract
The invention provides a quantum dot (QD) modified optical fiber-based biosensor which characterizes matrix metalloproteinase (MMP) enzyme activity at pain signaling sites in the central nervous system (CNS) in vivo. Related systems and peptide biomarker screening methods are also provided.
Claims30
A method for evaluating in vivo metalloproteinase (MMP) enzyme activity at a subject's central nervous system pain signaling site, the method comprising:
- (A) providing a quantum dot (QD) modified optical fiber-based biosensor system adapted for evaluation of metalloproteinase (MMP) enzyme activity at pain signaling sites in the central nervous system (CNS), said biosensor system comprising:
- (a) a multimode silica optical fiber comprising one or more quantum dots that (1) are bioconjugated on their surface to one or more peptides having an affinity for said metalloproteinase (MMP) enzyme, and (2) that are tethered to an insertion end of said multimode silica optical fiber by one or more silane coupling agents;
- and
- (b) a photon detector;
- wherein changes in photon emission resulting from interaction of said metalloproteinase (MMP) enzyme and peptides having an affinity for said metalloproteinase (MMP) enzyme are detected by said photon detector;
- (B) inserting the multimode silica optical fiber of the biosensor system comprising one or more quantum dots that (1) are bioconjugated on their surface to one or more peptides having an affinity for said metalloproteinase (MMP) enzyme, and (2) that are tethered to an insertion end of said multimode silica optical fiber by one or more silane coupling agents into an in-dwelling spinal catheter that is submerged into either the epidermal or intrathecal regions of the subject's central nervous system;
- and
- (C) measuring changes in photon emission resulting from interaction of said metalloproteinase (MMP) enzyme and peptides having an affinity for said metalloproteinase (MMP) enzyme, said changes having been detected by said photon detector.
5 more elements
A method for evaluating in vivo metalloproteinase (MMP) enzyme activity implicated in a subject's neuroimmune neuropathy in at least one site in said subject's central nervous system, the method comprising:
- (A) providing a quantum dot (QD) modified optical fiber-based biosensor system adapted for evaluation of metalloproteinase (MMP) enzyme activity implicated in neuroimmune neuropathy at sites in the central nervous system (CNS) of said subject, said biosensor system comprising:
- (a) a multimode silica optical fiber comprising one or more quantum dots that (1) are bioconjugated on their surface to one or more peptides having an affinity for said metalloproteinase (MMP) enzyme, and (2) that are tethered to an insertion end of said multimode silica optical fiber by one or more silane coupling agents;
- and
- (b) a photon detector;
- wherein changes in photon emission resulting from interaction of said metalloproteinase (MMP) enzyme and peptides having an affinity for said metalloproteinase (MMP) enzyme are detected by said photon detector;
- (B) inserting the multimode silica optical fiber of the biosensor system comprising one or more quantum dots that (1) are bioconjugated on their surface to one or more peptides having an affinity for said metalloproteinase (MMP) enzyme, and (2) that are tethered to an insertion end of said multimode silica optical fiber by one or more silane coupling agents into an in-dwelling spinal catheter that is submerged into either the epidermal or intrathecal regions of the subject's central nervous system;
- and
- (C) measuring changes in photon emission resulting from interaction of said metalloproteinase (MMP) enzyme and peptides having an affinity for said metalloproteinase (MMP) enzyme, said changes having been detected by said photon detector.
5 more elements
Dependencies are inferred from the claim text.