Light sheet microscope and method for operating same

A microscope comprising:

• an illumination objective, with a first optical axis, configured to produce a light sheet at least in part in a specimen plane;
• a detection objective, with a second optical axis, configured to detect light coming from the specimen plane;
• and
• an illumination optical unit for wide-field illumination of the specimen plane, the illumination optical unit comprising an illumination optical unit with a third optical axis;
• wherein the illumination objective and the detection objective are aligned relative to one another and relative to the specimen plane in such a way that the first optical axis and the second optical axis intersect in the specimen plane and form a substantially right angle therebetween;
• wherein the first optical axis and the second optical axis each form an angle which differs from zero with a reference axis orthogonal to the specimen plane;
• and
• wherein the detection objective is configured to detect both light from the light sheet, and light from the illumination optical unit, and the third optical axis of the illumination optical unit is directed substantially along the reference axis and is aligned at an angle which differs from zero in relation to the second optical axis of the detection objective.