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US 10,295,544 B2Granted
Apparatus and method for high trhoughput immunobloting
AssigneeQuanticision Diagnostics Inc.
InventorJiandi Zhang
Life of this patent
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Abstract
This disclosure provides high throughput immunoblot methods and apparatus for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The method yields highly sensitive and accurate results and is useful in analyze complex system including an antigen from cell or tissue lysate.
Claims19
A method for detecting an antigen, comprising:
- binding the antigen to a plurality of membrane units, wherein:
- each of the plurality of membrane units is individually compartmentalized to ensure signal emitted therefrom to be individually acquired, and
- each membrane unit is affixed to one of a plurality of corresponding supports,
- wherein said each corresponding support can hold said membrane unit;
- selecting a primary antibody that interacts with the antigen;
- binding the primary antibody to the antigen to form immunocomplexes so that each of the plurality of membrane units have immunocomplexes attached thereto;
- causing the immunocomplexes to emit signals;
- and
- detecting, from each of the plurality of membrane units, the signal emitted by the immunocomplexes attached thereto.
6 more elements
A method for immunoblot analysis, comprising:
- providing a multi-unit plate comprising a plurality of membrane units spaced away from one another, wherein:
- each of the plurality of membrane units is individually compartmentalized to
- ensure signal emitted therefrom to be individually acquired, and
- each membrane unit comprises a membrane;
- binding an antigen to one or more of the plurality of membrane units;
- selecting a primary antibody that interacts with the antigen;
- binding the primary antibody to the antigen to form immunocomplexes affixed to one or more of the plurality of membrane units, wherein the primary antibody is directly conjugated with a reporter enzyme, or indirectly conjugated with a reporter enzyme through one or more secondary antibody;
- and
- detecting from one or more of the membrane units light signals emitted by the reporter enzyme in the immunocomplexes.
6 more elements
Dependencies are inferred from the claim text.