Biorecognition elements for rapid detection of biocontamination
AssigneeThe United States of America as represened by the Secretary of the Air Force
InventorsOscar N Ruiz, Oksana Pavlyuk
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Abstract
A biorecognition element for detection of fuel biocontamination. The biorecognition element includes a sequence selected from SEQ. ID No. 10, SEQ. ID No. 13, SEQ. ID No. 22, SEQ. ID No. 27, SEQ. ID No. 31, SEQ. ID No.40, SEQ. ID No.67, SEQ. ID No. 68, SEQ. ID No. 69, SEQ. ID No. 70, SEQ. ID No. 71, and SEQ. ID No.72, an amine-functionalized quantum dot, a C-terminal, three-glycine plus cysteine linker, and a reporter molecule conjugated to the amine-functionalized quantum dot. Biocontaminants are labeled by the biorecognition element and observed via the reporter molecule of the biorecognition element.
Claims13
A biorecognition element for detection of microbial biocontamination, the biorecognition element comprising:
- SEQ. ID No. 10, SEQ. ID No. 13, SEQ. ID No. 22, SEQ. ID No. 27, SEQ. ID No. 31, SEQ. ID No. 40, SEQ. ID No. 67, SEQ. ID No. 68, SEQ. ID No. 69, SEQ. ID No. 70, SEQ. ID No. 71, or SEQ. ID No. 72;
- an amine-functionalized quantum dot;
- a C-terminal, three-glycine plus cysteine linker configured to cross-link to the amine-functionalized quantum dot;
- and
- a reporter molecule conjugated to the amine-functionalized quantum dot.
A method of detecting biocontamination, the method comprising:
- acquiring a sample;
- isolating microbes from the sample;
- labeling the microbes with a first reporter, wherein the first reporter is conjugated to a biorecognition element selected from the group consisting of:
- SEQ. ID No. 10, SEQ. ID No. 13, SEQ. ID No. 22, SEQ. ID No. 27, SEQ. ID No. 31, SEQ. ID No. 40, SEQ. ID No. 67, SEQ. ID No. 68, SEQ. ID No. 69, SEQ. ID No. 70, SEQ. ID No. 71, and SEQ. ID No. 72.
A biocontamination assay kit comprising:
- a biorecognition element selected from the group consisting of:
- SEQ. ID No. 10, SEQ. ID No. 13, SEQ. ID No. 22, SEQ. ID No. 27, SEQ. ID No. 31, SEQ. ID No. 40, SEQ. ID No. 67, SEQ. ID No. 68, SEQ. ID No. 69, SEQ. ID No. 70, SEQ. ID No. 71, and SEQ. ID No. 72;
- a C-terminal, three-glycine plus cysteine linker on the biorecognition element;
- an amine-functionalized quantum dot cross-linked to the three-glycine plus cysteine linker;
- and
- a reporter molecule conjugated to the amine-functionalized quantum dot.
Dependencies are inferred from the claim text.