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US 10,295,530 B2Granted
Functional assay for cancer recurrence and malignant potential
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsSandra S. McAllister, Zafira Castano
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Abstract
Embodiments herein provides an in vitro co-culture system comprising a population of cancer responder cells and a population of non-tumor cells wherein the cancer responder cells can convert to a malignant state and exhibit hallmark malignant phenotype when the cells are placed in a tumor supportive environment. The system is useful for prognosis evaluation of cancer recurrence, malignancy development, cancer drug screening and surveillance for resistance to cancer drug therapy.
Claims13
An assay comprising
- in vitro co-culturing a population of cancer responder cells with a population of non-tumor cells in the presence of plasma or platelets or exosomes, wherein the non-tumor cells are selected from the group consisting of bone marrow-derived circulating cells, buffy coat cells, peripheral circulating cells, and immune cells;
- and
- measuring for at least one malignant phenotype exhibited by the cancer responder cells;
- wherein at least one of (1) the population of cancer responder cells, (2) the population of non-tumor cells or (3) plasma, platelets, or exosomes is patient-derived and at least one of (1), (2) or (3) are standard positive plasma, platelets, exosomes or cell populations known to provide a tumor supportive environment.
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Dependencies are inferred from the claim text.