Hematoxylin precipitate cleaning method and system
AssigneeVentana Medical Systems, Inc.
InventorEdward E. Durrant
Life of this patent
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Abstract
A process for removing hematoxylin precipitate from a surface of an automated hematoxylin stainer, systems adapted for performing such processes. The process and systems use a hematein precipitate removal solution having a pH in the range of between about 8.9 and 10.5 and including a liquid oxidizing component and alkaline component. The hematein precipitate removal solution is placed in contact with a surface of the automated hematoxylin stainer, thereby dissolving any hematein precipitate deposited thereon.
Claims19
A method of removing hematein precipitate from an automated hematoxylin staining system comprising:
- providing a liquid oxidizing component comprising a peroxide and water and an alkaline component, wherein the liquid oxidizing component and the alkaline component are separate;
- combining the liquid oxidizing component and alkaline component in a container to form a hematein precipitate removal solution having a pH in the range of between about 8.9 and 10.5;
- placing the container of the hematein precipitate removal solution in a position to enable one or more hematoxylin dispensing component(s) of the automated hematoxylin staining system to receive and move the solution through an interior surface of the one or more hematoxylin dispensing component(s);
- activating the automated staining system to move the hematein precipitate removal solution into the interior surface of the hematoxylin dispensing component(s);
- contacting said interior surface for at least 30 minutes.
A method of removing a hematein precipitate from a surface of an automated hematoxylin staining system comprising contacting said surface with a hematein precipitate removal solution having a pH in the range of 8.9 to 10.5 for a period of time sufficient to dissolve the hematein precipitate, wherein said hematein precipitate removal solution comprises an aqueous peroxide solution combined with an alkaline component.
An automated hematoxylin staining system comprising:
- (a) a hematoxylin dispensing component having a surface susceptible to hematein precipitate buildup;
- and
- (b) a source of a hematein precipitate removal solution having a pH in the range of 8.9 to 10.5, said hematein precipitate removal solution comprising an aqueous peroxide solution combined with an alkaline component;
- wherein said automated slide stainer is configured to move the hematein precipitate removal solution in contact with the interior surface of the hematoxylin dispensing component for a sufficient period of time to dissolve the hematein precipitate buildup.
Dependencies are inferred from the claim text.