Respiratory infection assay

A quantitative uniplex method for detecting the presence of one or more of at least six respiratory infection-causing microorganisms in a sample or detecting the absence of said respiratory infection-causing microorganisms in said sample, said method comprising:

• A) applying a sample to a test card, wherein said test card comprises six discrete wells:
• 1) a first well that includes a first probe, for detecting the presence or absence of Respiratory syncytial virus (RSV A & B), wherein the first probe has a nucleic acid sequence that comprises the nucleic acid sequence TAGGCAATGCWGC (SEQ ID NO:
• 124);
• 2) a second well that includes a second probe, for detecting the presence or absence of Rhinoviruses, wherein the second probe has a nucleic acid sequence that comprises the nucleic acid sequence TCYGGGAYGGGACCRACTA (SEQ ID NO:
• 125);
• 3) a third well that includes a third probe, for detecting the presence or absence of Human Metapneumovirus (hMPV), wherein the third probe has a nucleic acid sequence that comprises the nucleic acid sequence TCAGCACCAGACACACC (SEQ ID NO:
• 126);
• 4) a fourth well that includes a fourth probe, for detecting the presence or absence of Influenza B (Flu B Quad), wherein the fourth probe has a nucleic acid sequence that comprises the nucleic acid sequence TCTGGTCATTGGRGCC (SEQ ID NO:
• 127);
• 5) a fifth well that includes a fifth probe, for detecting the presence or absence of Influenza A (Flu A CDC DC), wherein the fifth probe has a nucleic acid sequence that comprises the nucleic acid sequence CGCTCACTGGGCACGGT (SEQ ID NO:
• 128);
• 6) a sixth well that includes a sixth probe, for detecting the presence or absence of Influenza A subtype H5 (H5 FRET), wherein the sixth probe has a nucleic acid sequence that comprises the nucleic acid sequence AACTGRGTGTTCATTTTGT (SEQ ID NO:
• 129);
• B) allowing nucleic acid present in the sample to contact with the probes within said wells;
• C) detecting for the presence of bound nucleic acid complex in which sample nucleic acid has bound to one or more of said probes;
• wherein the presence of bound nucleic acid complex confirms that nucleic acid from one or more of said six respiratory infection-causing microorganisms is present within the sample, and wherein the absence of bound nucleic acid complex confirms that nucleic acid from all of said six respiratory infection-causing microorganisms is absent within the sample and wherein the probes comprise a detectable label and/or a detectable tag.