Method of extending DNA with telomerase and method of measuring telomerase activity
AssigneePanasonic Corporation
InventorsHidenobu Yaku, Daisuke Miyoshi
Life of this patent
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Abstract
The present disclosure provides a measurement method of telomerase activity with no occurrence of false-negative and high quantitative capability. In the measurement method of telomerase activity of the present disclosure, a solution containing telomerase as a sample solution is mixed with a solution containing primer DNA as a substrate for telomerase even as or after a solution containing non-primer DNA not as a substrate for telomerase is mixed therewith. Non-primer DNA can remove an effect of DNase in the sample solution and prevent occurrence of false-negative. Subsequently, the primer DNA is extended with the telomerase and the extended primer DNA is detected, through which telomerase activity is measured.
Claims9
A method for extending primer DNA with telomerase, the method comprising the steps of:
- (a) mixing non-primer DNA not as a substrate for telomerase with a solution containing contaminant DNase to provide a mixed solution, the non-primer DNA being selected to function as a substrate for the contaminant DNase;
- and
- (b) adding the primer DNA as a substrate for telomerase to the mixed solution to extend the primer DNA with the telomerase, while the non-primer DNA is being degraded by the DNase after the step (a).
A method for measuring an activity degree of telomerase, the method comprising the steps of:
- (a) mixing a solution containing contaminant DNase with non-primer DNA not as a substrate for telomerase to provide a mixed solution, the non-primer DNA being selected to function as a substrate for the contaminant DNase;
- (b) adding primer DNA as a substrate for telomerase to the mixed solution to extend the primer DNA with the telomerase, while the non-primer DNA is being degraded by the contaminant DNase after the step (a);
- and
- (c) determining an activity of the telomerase based on an extension degree of the extended primer DNA in the step (b).
A method for extending primer DNA with telomerase, the method comprising the steps of:
- (a) preparing a mixed solution containing non-primer DNA not as a substrate for telomerase and primer DNA as a substrate for telomerase, the non-primer DNA being selected to function as a substrate for contaminant DNase present in the solution;
- and
- (b) extending the primer DNA with the telomerase by adding a solution to the mixed solution while degrading the non-primer DNA with the DNase after the step (a).
A method for measuring an activity degree of telomerase in a solution containing contaminant DNase, the method comprising the steps of:
- (a) preparing a mixed solution containing M13DNA and primer DNA as a substrate for telomerase;
- (b) extending the primer DNA with the telomerase by adding a solution to the mixed solution while degrading the M13DNA with the DNase after the step (a);
- and
- (c) determining an activity of the telomerase based on an extension degree of the extended primer DNA in the step (b).
Dependencies are inferred from the claim text.