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US 10,294,520 B2Granted
Nicotiana nucleic acid molecules and uses thereof
AssigneeU.S. Smokeless Tobacco Company LLC
InventorsDongmei Xu, Mark T. Nielsen, Yanxin Shen
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Abstract
The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.
Claims18
A method comprising:
- obtaining genetic material from a Nicotiana plant, cured tobacco material, or a tobacco product;
- and detecting, in the genetic material, a non-naturally occurring mutation in an identified endogenous nicotine demethylase gene encoding a polypeptide having the sequence shown in SEQ ID NO:3, wherein the mutation reduces the expression or level of activity of said polypeptide having nicotine demethylase activity.
A method comprising:
- obtaining tissue from a Nicotiana plant, cured tobacco material, or a tobacco product;
- and detecting in the tissue from the Nicotiana plant, the cured tobacco material, or the tobacco product, a mutation in nucleotides 2010-2949 or 3947-4592 of SEQ ID NO:4.
A method comprising:
- obtaining genetic material from a Nicotiana plant, cured tobacco material, or a tobacco product;
- and detecting, in the genetic material, a non-naturally occurring null mutation in the first exon of an identified endogenous nicotine demethylase gene encoding a polypeptide having the sequence shown in SEQ ID NO:3, wherein the mutation reduces the expression or level of activity of said polypeptide having nicotine demethylase activity.
Dependencies are inferred from the claim text.