Method and apparatus for nucleic acid analysis

A method for nucleic acid analysis comprising:

• preparing a plurality of separate particles on a support substrate;
• fixing individual target nucleic acid fragments, of a group of different types of target nucleic acid fragments, on single ones of the particles in a one-to-one correspondence on the support substrate;
• and
• hybridizing a plurality of different nucleic acid molecules, which have different known base sequences and have been labeled with different fluorescent substances, with the group of the target nucleic acid fragments on the support substrate;
• and
• detecting the different fluorescent substances labeling the hybridized nucleic acid molecules;
• counting one or more luminescent spots corresponding to individual ones of the fluorescent substances for each of the different hybridized nucleic acid molecules;
• and
• calculating a ratio of the one or more luminescent spots for each of the different hybridized nucleic acid molecules to a total number of luminescent spots for all of the different hybridized nucleic acid molecules to evaluate an abundance thereof for each type of the target nucleic acid fragments,
• wherein the fixing of the individual target nucleic acid fragments and the hybridizing of the nucleic acid molecules are non-polymerase chain reaction (non-PCR) processes.