Method and apparatus for analyzing biomolecules by using oligonucleotide

A method for simultaneously analyzing a target protein and a target nucleic acid in a biological sample, comprising:

• (a) (i) reacting the target protein of the biological sample with an analysis ligand and forming a target protein-analysis ligand complex, the analysis ligand consisting of a ligand binding specifically to the target protein, and an oligonucleotide linked to the ligand, and
• (ii) producing a hybridization complex by hybridizing the target nucleic acid of the biological sample with an upstream oligonucleotide and a downstream oligonucleotide which are perfectly complementary to an upstream site and a downstream site of the target nucleic acid respectively, and making a double-stranded nucleic acid by extending a region between the upstream oligonucleotide and the downstream oligonucleotide on the hybridization complex, wherein the double-stranded nucleic acid comprises an extended nucleic acid strand which is complementary to the target nucleic acid, the upstream oligonucleotide and the downstream oligonucleotide;
• (b) making a mixture of the target protein-analysis ligand complex and the double-stranded nucleic acid;
• and
• (c) simultaneously analyzing the target protein and the target nucleic acid in the biological sample by detecting the oligonucleotide of the target protein-analysis ligand complex and the double-stranded nucleic acid, simultaneously, in the mixture of step (b).