Method for measuring ethanolamine phosphate
AssigneeHuman Metabolome Technologies, Inc.
InventorsTakushi Ohga, Yoshiaki Ohashi
Life of this patent
Estimating the term…
Abstract
To provide a method for simply measuring ethanolamine phosphate in a sample, and a reagent, kit, program and the like useful in the method. A measurement method of ethanolamine phosphate includes a first step of adding an enzyme, which can catalyze a reaction that forms acetaldehyde from ethanolamine phosphate, to a sample, and conducting a first enzymatic reaction to form acetaldehyde, phosphoric acid and ammonia; and a second step of quantifying at least one of the resultant acetaldehyde, phosphoric acid and ammonia to determine an amount of the ethanolamine phosphate in the measurement sample.
Claims7
A composition for screening a human patient having depression, comprising:
- a plasma sample of a human subject;
- and
- an ethanolamine phosphate-measuring reagent comprising an enzyme that catalyzes a reaction that forms acetaldehyde from ethanolamine phosphate,
- wherein the enzyme is a protein having an amino acid sequence region having at least 90% of homology with a GabT domain consisting of a following amino acid sequence:
- GXXXBADEBQXGFZRXG
- wherein X means an amino acid, B denotes a branched amino acid selected from the group consisting of I, L, and V, and Z signifies G or A, and
- the enzyme is derived from at least one source material selected from the group consisting of Pantoea, E. coli, Sulfolobus, Arabidopsis, Salmonella, Clostridium, Pseudomonas, Rhodococcus, Acinetobacter, Mycobacter, cattle, mice, larvacea, maize, and red peppers.
A composition for determining whether a human patient has depression, comprising:
- a plasma sample of the human patient;
- and
- an ethanolamine phosphate-measuring reagent comprising an enzyme that catalyzes a reaction that forms acetaldehyde from ethanolamine phosphate,
- wherein the enzyme is a protein having an amino acid sequence region having at least 90% of homology with a GabT domain consisting of a following amino acid sequence:
- GXXXBADEBQXGFZRXG
- wherein X means an amino acid, B denotes a branched amino acid selected from the group consisting of I, L, and V, and Z signifies G or A, and
- the enzyme is derived from at least one source material selected from the group consisting of Pantoea, E. coli, Sulfolobus, Arabidopsis, Salmonella, Clostridium, Pseudomonas, Rhodococcus, Acinetobacter, Mycobacter, cattle, mice, larvacea, maize, and red peppers.
Dependencies are inferred from the claim text.