Methods for biosynthesizing 1,3 butadiene
AssigneeInvista North America S.A.R.L.
InventorsAlex Van Eck Conradie, Adriana Leonora Botes
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Abstract
This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as, inter alia, a decarboxylating thioesterase, cytochrome P450, or dehydratases for the final enzymatic step.
Claims12
A method of butadiene synthesis, the method comprising introducing a first vinyl group into a first vinyl group acceptor compound of a 1,3-butadiene synthetic pathway using a dehydratase enzyme capable of introducing a first vinyl group into the first vinyl group acceptor compound, wherein the dehydratase enzyme capable of introducing the first vinyl group is linalool dehydratase (EC 4.2.1.127), 5-hydroxyvaleryl-CoA dehydratase from C. viride, or a dehydratase from species Aquincola tertiaricarbonis or Methylibium petroleiphilum PM1.
A method for the biosynthesis of butadiene comprising introducing a first vinyl group into a first vinyl group acceptor compound of a 1,3-butadiene synthetic pathway using a linalool dehydratase (EC 4.2.1.127), 5-hydroxyvaleryl-CoA dehydratase from C. viride, or a dehydratase from species Aquincola tertiaricarbonis or Methylibium petroleiphilum PM1, or a functional fragment or variant thereof having at least 25% of an activity and containing not more than 50 amino acid additions, deletions, or substitutions relative to a corresponding wild-type sequence of linalool dehydratase (EC 4.2.1.127), 5-hydroxyvaleryl-CoA dehydratase from C. viride, or a dehydratase from species Aquincola tertiaricarbonis or Methylibium petroleiphilum PM1.
Dependencies are inferred from the claim text.