Back to results
US 10,294,492 B2Granted
Stable episomes based on non-integrative lentiviral vectors
AssigneeFundacióN Centro Nacional De Investigaciones Cariovasculares Carlos III (Cnic)
InventorsJuan Carlos Ramírez Martínez, Raúl Torres Ruiz, Aida García Torralba
Life of this patent
Estimating the term…
Abstract
The invention relates to non-integrative lentiviral vectors and their use for the stable transgenesis of both dividing and no-dividing eukaryotic cells. The invention also provides methods for obtaining these vectors, the use of these vectors for the production of recombinant lentiviruses, and the use of these recombinant lentiviruses for obtaining a cell able to stably produce a product of interest.
Claims20
A polynucleotide comprising
- (i) a first long terminal repeat derived from a lentivirus,
- (ii) an eukaryotic origin of replication selected from the group consisting of the origins of replication having the sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3 and a functionally equivalent variant thereof, wherein the functionally equivalent variant has at least 70% of sequence identity across the whole length of the sequence with the sequences SEQ ID NO;
- 1, SEQ ID NO:
- 2 or SEQ ID NO:
- 3,
- (iii) a scaffold/matrix attachment region selected from the group consisting of the scaffold/matrix attachment regions having the sequence of SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6 and a functionally equivalent variant thereof, wherein the functionally equivalent variant has at least 70% of sequence identity across the whole length of the sequence with the sequences SEQ ID NO;
- 4, SEQ ID NO:
- 5 or SEQ ID NO:
- 6, and
- (iv) a second long terminal repeat derived from said lentivirus, wherein said eukaryotic origin of replication and said scaffold/matrix attachment region are located between said first and second long terminal repeats.
13 more elements
Dependencies are inferred from the claim text.