Soybean resistant to cyst nematodes
AssigneesBoard of Trustees of Southern Illinois University, The Curators of the University of Missouri
InventorsKhalid Meksem, Shiming Liu, Pramod Kaitheri Kandoth, Melissa G. Mitchum, David Lightfoot
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Abstract
A transgenic soybean plant or parts thereof, resistant to soybean cyst nematodes, transformed to express Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof. Also provided is a method of making such a plant. Also provided is an artificial DNA construct encoding Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof.
Claims19
A transgenic soybean plant resistant to soybean cyst nematode (SCN), or a seed, plant part, or progeny thereof, the soybean plant transformed with an artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription:
- (a) a promoter that functions in a soybean plant;
- (b) a transcribable nucleic acid molecule comprising
- (i) a nucleotide sequence at least 99% identical to SEQ ID NO:3, wherein said nucleotide sequence comprises a C163225G mutation, and wherein said nucleotide sequence encodes a polypeptide having soluble NSF-attachment protein (SNAP) activity;
- (ii) a nucleotide sequence encoding a polypeptide at least 99% identical to SEQ ID NO:6, wherein said polypeptide comprises a D208E mutation, and wherein said polypeptide has SNAP activity, or;
- (iii) a nucleotide sequence which is the full length complement of (i) or (ii);
- and
- (c) a transcriptional termination sequence;
- wherein the promoter is heterologous to the transcribable nucleic acid molecule, and the transgenic soybean plant exhibits increased SCN resistance compared to a control transgenic soybean plant expressing SEQ ID NO:3.
An artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription:
- (a) a promoter that functions in a soybean plant;
- (b) a transcribable nucleic acid molecule comprising
- (i) a nucleotide sequence at least 99% identical to SEQ ID NO:3, wherein said nucleotide sequence comprises a C163225G mutation, and wherein said nucleotide sequence encodes a polypeptide having SNAP activity,
- (ii) a nucleotide sequence encoding a polypeptide least 99% identical to SEQ ID NO:6, wherein said polypeptide comprises a D208E mutation, and wherein said polypeptide has SNAP activity, or;
- (iii) a nucleotide sequence which is the full length complement of (i) or (ii);
- and
- (c) a transcriptional termination sequence.
Dependencies are inferred from the claim text.