Plant promoter and 3′ UTR for transgene expression
AssigneeDow AgroSciences LLC
InventorsManju Gupta, Sara Bennett, Andrew F. Worden
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Abstract
This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin C9 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin C9 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.
Claims18
A nucleic acid vector comprising a 3′ UTR operably linked to:
- a) a polylinker sequence;
- b) a heterologous coding sequence;
- or
- c) a combination of a) and b), wherein said 3′ UTR comprises a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:1.
A plant comprising a polynucleotide sequence inserted into the genome of said plant, wherein the polynucleotide sequence has at least 97% sequence identity with SEQ ID NO:1 operably linked to a transgene.
A method for producing a transgenic plant cell, the method comprising the steps of:
- a) transforming a plant cell with a gene expression cassette comprising a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:1 operably linked to at least one heterologous coding sequence;
- b) isolating the transformed plant cell comprising the gene expression cassette;
- and
- c) producing a transgenic plant cell comprising the polynucleotide sequence operably linked to at least one heterologous coding sequence, wherein the polynucleotide sequence operably linked to at least one heterologous coding sequence is stably integrated within the genome of the transgenic plant cell.
Dependencies are inferred from the claim text.