Microbial production of renewable glycolate
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Zheng-Jun Li, Brian Pereira
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Abstract
Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.
Claims18
An Escherichia coli cell engineered to produce glycolate, wherein the cell comprises a deletion of a xylB gene, recombinantly expresses a dte gene from Pseudomonas cichorii that is codon-optimized for E. coli, recombinantly expresses a fucA gene from E. coli, recombinantly expresses a fucK gene from E. coli, recombinantly expresses an aldA gene from E. coli, and recombinantly expresses a ycdW gene from E. coli,
- wherein one or more of the recombinantly expressed genes are under the control of one or more promoters,
- wherein the one or more promoters is not a native promoter, and
- wherein the cell has higher production of glycolate relative to a wild type cell.
An Escherichia coli cell engineered to produce glycolate,
- wherein the cell comprises a deletion of a xylB gene, recombinantly expresses a dte gene from Pseudomonas cichorii that is codon-optimized for E. coli, recombinantly expresses a fucA gene from E. coli, recombinantly expresses a fucK gene from E. coli, and recombinantly expresses an aldA gene from E. coli,
- wherein the cell further comprises a deletion of a fucO gene.
Dependencies are inferred from the claim text.