3-epimerase
AssigneeTate & Lyle Ingredients Americas LLC
InventorsRyan David Woodyer, Richard W. Armentrout
Life of this patent
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Abstract
The disclosure provides a method of producing allulose by contacting a protein having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 6 with a fructose substrate, wherein the protein has allulose 3-epimerase activity, and at least partially purifying the allulose. The disclosure also provides a method of producing allulose by providing a vector comprising a nucleic acid molecule having a polynucleotide sequence encoding a protein having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 6, wherein the protein has allulose 3-epimerase activity, synthesizing the protein having allulose 3-epimerase activity, contacting fructose with the protein having allulose 3-epimerase activity, and partially purifying the allulose produced.
Claims20
A method of producing allulose comprising:
- (a) contacting a protein with a fructose substrate under conditions such that the fructose substrate is converted into allulose, wherein the protein comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 6, wherein the protein has allulose 3-epimerase activity, and
- (b) at least partially purifying the allulose.
A method of producing allulose comprising the steps of:
- i) providing a vector comprising a nucleic acid molecule having a polynucleotide sequence encoding a protein comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 6, wherein the protein has allulose 3-epimerase activity;
- ii) synthesizing the protein having allulose 3-epimerase activity encoded by the polynucleotide sequence;
- iii) contacting fructose with the protein having allulose 3-epimerase activity and maintaining the fructose and the protein under conditions to permit the conversion of fructose to allulose;
- and
- iv) at least partially purifying the allulose produced in step iii).
Dependencies are inferred from the claim text.